Detail Information for IndEnz0009000294
IED ID IndEnz0009000294
Enzyme Type ID chitinase000294
Protein Name Basic endochitinase
EC 3.2.1.14
Fragment
Gene Name CHI14
Organism Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Solanoideae Solaneae Solanum Solanum subgen. Lycopersicon Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Enzyme Sequence LSQNISSLISKNLFERILVHRNDRACGAKGFYTYEAFITATKTFAAFGTTGDTNTRNKEIAAFLAQTSHETTGGWATAPDGPYSWGYCYNREQGSPGDYCASSQQWPCAPGKKYFGRGPIQISYNYNYGAAGSAIGVNLLNNPDLVANDAVVSFKTALWFWMTAQQPKPSAHDVITGRWSPSVADSAPGRVPGFGVITNIINGGMECNSGSNALMDNRIGFYRRYCQILGVDPGNNLDCANQRPFG
Enzyme Length 246
Uniprot Accession Number Q05537
Absorption
Active Site ACT_SITE 70; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
DNA Binding
EC Number 3.2.1.14
Enzyme Function FUNCTION: Defense against chitin-containing fungal pathogens.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Disulfide bond (3); Non-terminal residue (1)
Keywords Carbohydrate metabolism;Chitin degradation;Disulfide bond;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Reference proteome;Secreted
Interact With
Induction INDUCTION: By fungal infection.
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 26,686
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda