IED ID | IndEnz0009000297 |
Enzyme Type ID | chitinase000297 |
Protein Name |
26 kDa endochitinase 1 EC 3.2.1.14 |
Gene Name | |
Organism | Hordeum vulgare (Barley) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae BOP clade Pooideae Triticodae Triticeae Hordeinae Hordeum Hordeum vulgare (Barley) |
Enzyme Sequence | MRAFVLFAVVAMAATMAVAEQCGSQAGGATCPNCLCCSRFGWCGSTPYCGDGCQSQCSGCGGGSTPVTPTPSGGGGVSSIVSRALFDRMLLHRNDGACQAKGFYTYDAFVAAASAFRGFGTTGGTDTRKREVAAFLAQTSHETTGGWATAPDGAFAWGYCFKQERGATSNYCTPSAQWPCAPGKSYYGRGPIQLSHNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTAMWFWMTAQAPKPSSHAVITGQWSPSGTDRAAGRVPGFGVITNIVNGGIECGHGQDSRVADRIGFYKRYCDILGVGYGNNLDCYSQRPFA |
Enzyme Length | 318 |
Uniprot Accession Number | P11955 |
Absorption | |
Active Site | ACT_SITE 142; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; |
DNA Binding | |
EC Number | 3.2.1.14 |
Enzyme Function | FUNCTION: Defense against chitin-containing fungal pathogens. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Chain (1); Disulfide bond (7); Domain (1); Signal peptide (1) |
Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Signal |
Interact With | |
Induction | INDUCTION: By ethylene. |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 33,402 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda | 3.2.1.14; |