| IED ID | IndEnz0009000299 |
| Enzyme Type ID | chitinase000299 |
| Protein Name |
Endochitinase EP3 EC 3.2.1.14 Chitinase class IV AtchitIV Protein HOMOLOG OF CARROT EP3-3 CHITINASE AtEP3 |
| Gene Name | EP3 CHIV At3g54420 T12E18.110 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MLTPTISKSISLVTILLVLQAFSNTTKAQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAPPPANGVSVAEIVTQEFFNGIISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYIEEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVATDPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQLGVDPGNNLTC |
| Enzyme Length | 273 |
| Uniprot Accession Number | Q9M2U5 |
| Absorption | |
| Active Site | ACT_SITE 136; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P29022 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; Evidence={ECO:0000250|UniProtKB:P29022}; |
| DNA Binding | |
| EC Number | 3.2.1.14 |
| Enzyme Function | FUNCTION: Probably involved in hypersensitive reaction upon Xanthomonas campestris infection. {ECO:0000269|PubMed:9426222}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 4.8. {ECO:0000269|PubMed:11525512}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (4); Domain (1); Erroneous initiation (1); Glycosylation (4); Region (1); Sequence conflict (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Chitin degradation;Chitin-binding;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Plant defense;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | INDUCTION: Accumulates rapidly and transiently in leaves after inoculation with Xanthomonas campestris (PubMed:9426222). Slightly repressed by wounding (PubMed:19420714). {ECO:0000269|PubMed:19420714, ECO:0000269|PubMed:9426222}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12535341; 12805589; 14595688; 15181213; 15200641; 16021335; 16258011; 16489130; 16553894; 16829591; 17181774; 17194768; 18603616; 18650403; 20736450; 23682113; 28724081; |
| Motif | |
| Gene Encoded By | |
| Mass | 29,436 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |