| IED ID | IndEnz0009000320 |
| Enzyme Type ID | chitinase000320 |
| Protein Name |
Cortical fragment-lytic enzyme CFLE EC 3.2.1.- Spore germination protein Spore peptidoglycan N-acetylglucosaminidase |
| Gene Name | sleL yaaH BSU00160 |
| Organism | Bacillus subtilis (strain 168) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
| Enzyme Sequence | MQIYVVKQGDTLSAIASQYRTTTNDITETNEIPNPDSLVVGQTIVIPIAGQFYDVKRGDTLTSIARQFNTTAAELARVNRIQLNTVLQIGFRLYIPPAPKRDIESNAYLEPRGNQVSENLQQAAREASPYLTYLGAFSFQAQRNGTLVAPPLTNLRSITESQNTTLMMIITNLENQAFSDELGRILLNDETVKRRLLNEIVENARRYGFRDIHFDFEYLRPQDREAYNQFLREARDLFHREGLEISTALAPKTSATQQGRWYEAHDYRAHGEIVDFVVLMTYEWGYSGGPPQAVSPIGPVRDVIEYALTEMPANKIVMGQNLYGYDWTLPYTAGGTPARAVSPQQAIVIADQNNASIQYDQTAQAPFFRYTDAENRRHEVWFEDARSIQAKFNLIKELNLRGISYWKLGLSFPQNWLLLSDQFNVVKKTFR |
| Enzyme Length | 431 |
| Uniprot Accession Number | P37531 |
| Absorption | |
| Active Site | ACT_SITE 217; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | BINDING 218; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258; BINDING 406; /note=Chitooligosaccharide; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.2.1.- |
| Enzyme Function | FUNCTION: N-acetylglucosaminidase involved in cortex peptidoglycan degradation during germination. Cleaves only partially degraded spore peptidoglycans. Recognizes muramic acid delta-lactam residues specific to spore peptidoglycans. {ECO:0000250|UniProtKB:Q9K3E4}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Binding site (2); Chain (1); Domain (3); Erroneous initiation (2); Region (3) |
| Keywords | Direct protein sequencing;Germination;Glycosidase;Hydrolase;Reference proteome;Repeat |
| Interact With | |
| Induction | INDUCTION: Expression is regulated by the sporulation transcription factor sigma E. {ECO:0000269|PubMed:10419957}. |
| Subcellular Location | SUBCELLULAR LOCATION: Spore coat {ECO:0000269|PubMed:19933362}. Note=Probably present in the inner coat. {ECO:0000269|PubMed:19933362}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 49,140 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |