| IED ID | IndEnz0009000323 |
| Enzyme Type ID | chitinase000323 |
| Protein Name |
Cortical fragment-lytic enzyme CFLE EC 3.2.1.- Spore peptidoglycan N-acetylglucosaminidase |
| Gene Name | sleL BAS3402 |
| Organism | Bacillus anthracis |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus group Bacillus anthracis |
| Enzyme Sequence | MIQIVTVRSGDSVYSLASKYGSTPDEIVKDNGLNPAETLVVGQALIVNTKGNNYYVQPGDSLYRISQTYNVPLASLAKVNNLSLKSILHVGQQLYIPKGTKRAVESIAYLQPSTIPIKESLVNATRAINPFLTYLAYFSFEAKRDGTLKEPTETAKIANIATQGNTIPMLVITNIENGNFSADLTSVILRDATIQNKFITNILQTAEKYGMRDIHFDFESVAPEDREAYNRFLRNVKTRLPSGYTLSTTLVPKTSSNQKGKFFETHDYKAQGQIVDFVVIMTYDWGWQGGPPMAISPIGPVKEVLQYAKSQMPPQKIMMGQNLYGFDWKLPFKEGNPPAKAISSVAAVALARKYNVPIRYDFTAQAPHFNYFDENGVQHEVWFEDSRSVQSKFNLMKEQGIGGISYWKIGLPFPQNWRLLVENFTITKKG |
| Enzyme Length | 430 |
| Uniprot Accession Number | P0DPJ9 |
| Absorption | |
| Active Site | ACT_SITE 219; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01258 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.2.1.- |
| Enzyme Function | FUNCTION: N-acetylglucosaminidase involved in cortex peptidoglycan degradation during germination (PubMed:18835992, PubMed:22343356). Cleaves only partially degraded spore peptidoglycans. Recognizes muramic acid delta-lactam residues specific to spore peptidoglycans (PubMed:22343356). {ECO:0000269|PubMed:18835992, ECO:0000269|PubMed:22343356}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (3) |
| Keywords | Germination;Glycosidase;Hydrolase;Repeat |
| Interact With | |
| Induction | INDUCTION: Expressed during sporulation. {ECO:0000269|PubMed:18835992}. |
| Subcellular Location | SUBCELLULAR LOCATION: Forespore {ECO:0000269|PubMed:22343356}. Note=Localizes to the coat or cortex of the endospore. {ECO:0000269|PubMed:22343356}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 48,169 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |