| IED ID |
IndEnz0009000332 |
| Enzyme Type ID |
chitinase000332 |
| Protein Name |
Toxin subunit YenC2
|
| Gene Name |
yenC2 |
| Organism |
Yersinia entomophaga |
| Taxonomic Lineage |
cellular organisms
Bacteria
Proteobacteria
Gammaproteobacteria
Enterobacterales
Yersiniaceae
Yersinia
Yersinia entomophaga
|
| Enzyme Sequence |
MDIQLFSKTPSVTVFDNRGLSVRDIAYRRHPDTPKVTEECITYHQFDFRGFLAQSLDPRLNHKEVTNFSYLTDLNGNIIYTQSVDAGNTLVLNDTEGRSVIAMTNISRGENGKDDLSLAVTRTFQYENAPLPGRPLSVTEQVNGENARITEHFVYAGNTPQEKNLNLAGQCVSYYDAAGLIQTDSVSLTGKPLSVSRKLLKNLDDTNILADWQGNDTSAWNSLLATEIYTTVTRTDAAGAVLTTIDAVGNQQRVAFDIAGQLSASWLTLKGGQEQVIIKVLTYSAAGQKLREEGGNGVVTTYTYEAETQRLIGIKTERPNGHAAGAKVLQDLRYEYDPVGNVLSITNDAEETRFWRNQKVVPENAYRYDSLYQLVSASGREVAGAGQQGSDLPSPLVPLPSDSSVYTNYTRTYTYDSAGNLMRIRHSAPATNNNYTLNITVSERSNRGVMSSLTENPADVDALFTASGSQKCLQQGQSLIWTPRGELRTVLLVARGETADDSESYRYDGSSQRILKISSQQTNHSARVQRALYLPGLEWRTMTGGVAEAENLQVICIGEAGRAQVRVLHWESGKPDGIINDQIRWSYDNLTCSSGLEVDGDGLVISMEEYYPYGGTAVWAARSHIETAYKTVRYSGKERDATGLYYYGFRYYQPWAGRWLSADPAGTVDGLNLYRMVRNNPLRLTDPDGMAPLDWLDLDTTNASRDIVKAIYQLNQIDGPHRGVRDTYQRMTESTGMILQETLNNEAVLKGIKQKDKEKKSRGMKFTNSKLKTYAAHAGVLNTLQPDPVYKDGFLNLPGSLGNKNTFPGVELIEDKVKPSLSQYHPDKLGKSQRWKPESSLGYYRVADTEAFITGIRSQYKSSGTDLHAVVEGRIRDHLLANNNVLPKMAGIAGLHAEVQALNYIISNPDIEGGNAERLNGSYIFTQRLVGDVNQDFPACYNCSGIISGLENVMTGRVNNDVRLKRRKSF |
| Enzyme Length |
970 |
| Uniprot Accession Number |
B6A882 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
ACTIVITY REGULATION: Toxin complex is secreted when grown at 25 degrees Celsius or less; at higher temperatures the proteins are present intracellularly but not secreted. {ECO:0000269|PubMed:21278295}. |
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Part of an orally active toxin complex (TC) with strong insecticidal effects on larvae of the Coleoptera Costelytra zealandica, Acrossidius tasmania and Adoryphorus couloni and some Lepidoptera larvae (PubMed:21278295). The TC has an endochitinase activity (PubMed:21278295) (Probable). {ECO:0000269|PubMed:21278295, ECO:0000305|PubMed:22158901}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Beta strand (47); Chain (1); Helix (9); Mutagenesis (3); Region (2); Repeat (9); Site (1); Turn (10) |
| Keywords |
3D-structure;Autocatalytic cleavage;Repeat;Secreted;Virulence |
| Interact With |
B6A880 |
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21278295}. Note=Secreted when grown at 25 degrees Celsius or less, but not when grown at 30 or 37 degrees Celsius. {ECO:0000269|PubMed:21278295}. |
| Modified Residue |
|
| Post Translational Modification |
|
| Signal Peptide |
|
| Structure 3D |
X-ray crystallography (1) |
| Cross Reference PDB |
4IGL;
|
| Mapped Pubmed ID |
- |
| Motif |
|
| Gene Encoded By |
|
| Mass |
107,447 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|