| IED ID | IndEnz0009000347 |
| Enzyme Type ID | chitinase000347 |
| Protein Name |
FYVE zinc finger domain protein UPA1 PAM2 domain-containing protein UPA1 |
| Gene Name | UPA1 UMAG_12183 |
| Organism | Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Ustilaginomycotina Ustilaginomycetes Ustilaginales Ustilaginaceae Ustilago Ustilago maydis (Corn smut fungus) Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) |
| Enzyme Sequence | MTIPDPANIIHNDAGTASPHHIWADVGDSTSSSQHEATRSRSDDANGGASASMHAPQHVKANRAAQPTYDSSDLPSFGLSARLTRDSSSFGSKPSSSASDSRRPKFAPYEAENLWATSSTTSHPSKASQSTLSPNASVFKPSRSLQPNHFEPHAVANVHDFDDPLNSAYSSDTVSPRPDHAPLDHEQPQQPSALDPVAVSKVEEQRGDHSIPHQNGLVSAQAQTASDAVSTSKYTTEAADQEEDQDDFVYPGADSPSSGQAAVQDEQQAVTDSQTTKSLTKQESDPEASSTSLSAPAEAEHIVVGSAAEQSLTSSAPAETAVHIDYDTLAQLCSRGPLSDLQSFFHTAQESGLSMFSLSNDPNPGNGLVPLHFAAKDGKTDIVRWLITQAGAIVEMEDREGETALHKAAMAGKLSVASLLLSHGADANAQDADGWTALHNACSRGYLDLVRLLVDRGHAQIDVQGGRGAWTPLMNAASKGHLPVVRHLTAKYHADPFVRNAAGETAFDVAAATFEVYICEILERYEAERWNASKFTTSSPSRSGAIVPGRGPYEPLALHTTIPVILHENQRLDTRLQTLALNGGKPRWSSSSAARAHKPDRRSPSSMPPGPLAPSRTRHVPMRQDDVGLPTRSLPYKLRLRSRVGPAAARRRAAALAAQHTPNPQDCHDDDLASTPTPESVLQARRGTSSVNGASAQHADAESSHFWLCEWQLDTTHPLVDVEHGWQYAQSFDALDDKWSSQPPPPLERLLEGRGLSASVTRAITGGAGFANAQAEQEISSSSWVRRRRWIRVLRRRLDIEFGDDLEACEGATGAGAEHLVLSSESQSNGDGSHGLSTAAIMAAQEAAKSECSQLGPDADYVSRAKALAGPSAASGATPADAMGADRDELARRIARLVMANTELRAAFEDDDVERRSRAEELRKEYALQLGQLREAAGLDEDEDEDAADDDDDEFIYPNSYKDDGASVFTRLVNGETSGTLSRPSLSQRQSSAASMLRNSVAPSEAGTSLAAARSADLAANREFRVPTNEAPNKVVLRHGPTMREQNLQPQWQRDEEAKDCIGCGRHFTFFLRKHHCRRCGRIFCDACSSKRAQLRMAELVVDPSLPSMAASEVLAPTRVCNGCHAELQLPPQLQNMRGADAMMAASRSRGADEVSGRSILETQLEDGAFRSTLAPPSDVSSRASELTECPVCSTTLSALGGSEEQEAHVRNCLENGGGGSMQGGRYLVYKLPEDSPIVGKECSICMEDFVANSTIARLPCLCYFHRGCIDSWFKRGRECPVHARDW |
| Enzyme Length | 1287 |
| Uniprot Accession Number | A0A0D1E015 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: FYVE zinc finger domain protein that functions in endosomal targeting and transport of mRNAs, as well as associated ribosomes (PubMed:25985087, PubMed:28422978). The endosomal mRNA transport regulates polarity of the infectious hyphae by transporting a broad spectrum of cargo mRNAs from the nucleus to cell poles (PubMed:25985087). Involved in chitinase CTS1 secretion (PubMed:25985087). Dispensable for general endosomal functions but crucial for endosomal recruitment of RRM4 (PubMed:25985087). {ECO:0000269|PubMed:25985087, ECO:0000269|PubMed:28422978}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Metal binding (8); Motif (3); Repeat (4); Zinc finger (2) |
| Keywords | ANK repeat;Cytoplasm;Cytoskeleton;Endosome;Metal-binding;Reference proteome;Repeat;Transferase;Transport;Ubl conjugation pathway;Zinc;Zinc-finger;mRNA transport |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000269|PubMed:25985087}. Endosome {ECO:0000269|PubMed:25985087}. Note=Shuttles with endosomes along microtubules (PubMed:25985087). the FYVE domain mediates endosomal localization and endosomal targeting is crucial for its function during polar growth and CTS1 secretion (PubMed:25985087). {ECO:0000269|PubMed:25985087}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 128..144; /note=PAM2; /evidence=ECO:0000269|PubMed:25985087; MOTIF 239..253; /note=PAM2L 1; /evidence=ECO:0000269|PubMed:25985087; MOTIF 941..960; /note=PAM2L 2; /evidence=ECO:0000269|PubMed:25985087 |
| Gene Encoded By | |
| Mass | 138,533 |
| Kinetics | |
| Metal Binding | METAL 1061; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00091; METAL 1064; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00091; METAL 1077; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00091; METAL 1080; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00091; METAL 1085; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00091; METAL 1088; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00091; METAL 1121; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00091; METAL 1124; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00091 |
| Rhea ID | |
| Cross Reference Brenda |