| IED ID | IndEnz0010000002 |
| Enzyme Type ID | esterase000002 |
| Protein Name |
Hemagglutinin-esterase-fusion glycoprotein HEF EC 3.1.1.53 Cleaved into: Hemagglutinin-esterase-fusion glycoprotein chain 1 HEF1 ; Hemagglutinin-esterase-fusion glycoprotein chain 2 HEF2 |
| Gene Name | HE |
| Organism | Influenza C virus (strain C/Johannesburg/1/1966) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Negarnaviricota Polyploviricotina Insthoviricetes Articulavirales Orthomyxoviridae Gammainfluenzavirus Influenza C virus Influenza C virus (strain C/Johannesburg/1/1966) |
| Enzyme Sequence | MFFSLLLVLGLTEAEKIKICLQKQVNSSFSLHNGFGGNLYATEEKRMFELVKPKAGASVLNQSTWIGFGDSRTDKSNSAFPRSADVSAKTADKFRFLSGGSLMLSMFGPPGKVDYLYQGCGKHKVFYEGVNWSPHAAINCYRKNWTDIKLNFQKNIYELASQSHCMSLVNALDKTIPLQVTAGTAGNCNNSFLKNPALYTQEVKPSENKCGKENLAFFTLPTQFGTYECKLHLVASCYFIYDSKEVYNKRGCDNYFQVIYDSFGKVVGGLDNRVSPYTGNSGDTPTMQCDMLQLKPGRYSVRSSPRFLLMPERSYCFDMKEKGPVTAVQSIWGKGRESDYAVDQACLSTPGCMLIQKQKPYIGEADDHHGDQEMRELLSGLDYEARCISQSGWVNETSPFTEKYLLPPKFGRCPLAAKEESIPKIPDGLLIPTSGTDTTVTKPKSRIFGIDDLIIGVLFVAIVETGIGGYLLGSRKESGGGVTKESAEKGFEKIGNDIQILKSSINIAIEKLNDRISHDEQAIRDLTLEIENARSEALLGELGIIRALLVGNISIGLQESLWELASEITNRAGDLAVEVSPGCWIIDNNICDQSCQNFIFKFNETAPVPTIPPLDTKIDLQSDPFYWGSSLGLAITATISLAALVISGIAICRTK |
| Enzyme Length | 655 |
| Uniprot Accession Number | P07975 |
| Absorption | |
| Active Site | ACT_SITE 71; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_04072; ACT_SITE 366; /note=Charge relay system; /evidence=ECO:0000255|HAMAP-Rule:MF_04072; ACT_SITE 369; /note=Charge relay system; /evidence=ECO:0000255|HAMAP-Rule:MF_04072 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + N-acetyl-9-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate; Xref=Rhea:RHEA:22600, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28999, ChEBI:CHEBI:30089, ChEBI:CHEBI:35418; EC=3.1.1.53; Evidence={ECO:0000255|HAMAP-Rule:MF_04072}; CATALYTIC ACTIVITY: Reaction=H2O + N-acetyl-4-O-acetylneuraminate = acetate + H(+) + N-acetylneuraminate; Xref=Rhea:RHEA:25564, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29006, ChEBI:CHEBI:30089, ChEBI:CHEBI:35418; EC=3.1.1.53; Evidence={ECO:0000255|HAMAP-Rule:MF_04072}; |
| DNA Binding | |
| EC Number | 3.1.1.53 |
| Enzyme Function | FUNCTION: Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. {ECO:0000255|HAMAP-Rule:MF_04072}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (32); Chain (3); Disulfide bond (7); Glycosylation (7); Helix (13); Mutagenesis (5); Natural variant (4); Region (9); Signal peptide (1); Topological domain (2); Transmembrane (1); Turn (6) |
| Keywords | 3D-structure;Disulfide bond;Fusion of virus membrane with host endosomal membrane;Fusion of virus membrane with host membrane;Glycoprotein;Hemagglutinin;Host cell membrane;Host membrane;Host-virus interaction;Hydrolase;Membrane;Signal;Transmembrane;Transmembrane helix;Viral attachment to host cell;Viral envelope protein;Viral penetration into host cytoplasm;Virion;Virus endocytosis by host;Virus entry into host cell |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Virion membrane {ECO:0000255|HAMAP-Rule:MF_04072}; Single-pass type I membrane protein {ECO:0000255|HAMAP-Rule:MF_04072}. Host cell membrane {ECO:0000255|HAMAP-Rule:MF_04072}; Single-pass type I membrane protein {ECO:0000255|HAMAP-Rule:MF_04072}. |
| Modified Residue | |
| Post Translational Modification | PTM: In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease. {ECO:0000255|HAMAP-Rule:MF_04072}. |
| Signal Peptide | SIGNAL 1..14; /evidence=ECO:0000255|HAMAP-Rule:MF_04072 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 1FLC; 6WKO; |
| Mapped Pubmed ID | 34010634; |
| Motif | |
| Gene Encoded By | |
| Mass | 72,131 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22600; RHEA:25564 |
| Cross Reference Brenda | 3.1.1.53; |