IED Industry Enzyme Database

Detail Information for IndEnz0010000011
IED ID IndEnz0010000011
Enzyme Type ID esterase000011
Protein Name Bile salt-activated lipase
BAL
EC 3.1.1.13
EC 3.1.1.3
EC 3.1.1.6
Bile salt-stimulated lipase
BSSL
Bucelipase
Carboxyl ester lipase
Cholesterol esterase
Pancreatic lysophospholipase
Sterol esterase
Gene Name CEL BAL
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MGRLQLVVLGLTCCWAVASAAKLGAVYTEGGFVEGVNKKLGLLGDSVDIFKGIPFAAPTKALENPQPHPGWQGTLKAKNFKKRCLQATITQDSTYGDEDCLYLNIWVPQGRKQVSRDLPVMIWIYGGAFLMGSGHGANFLNNYLYDGEEIATRGNVIVVTFNYRVGPLGFLSTGDANLPGNYGLRDQHMAIAWVKRNIAAFGGDPNNITLFGESAGGASVSLQTLSPYNKGLIRRAISQSGVALSPWVIQKNPLFWAKKVAEKVGCPVGDAARMAQCLKVTDPRALTLAYKVPLAGLEYPMLHYVGFVPVIDGDFIPADPINLYANAADIDYIAGTNNMDGHIFASIDMPAINKGNKKVTEEDFYKLVSEFTITKGLRGAKTTFDVYTESWAQDPSQENKKKTVVDFETDVLFLVPTEIALAQHRANAKSAKTYAYLFSHPSRMPVYPKWVGADHADDIQYVFGKPFATPTGYRPQDRTVSKAMIAYWTNFAKTGDPNMGDSAVPTHWEPYTTENSGYLEITKKMGSSSMKRSLRTNFLRYWTLTYLALPTVTDQEATPVPPTGDSEATPVPPTGDSETAPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDAGPPPVPPTGDSGAPPVPPTGDSGAPPVTPTGDSETAPVPPTGDSGAPPVPPTGDSEAAPVPPTDDSKEAQMPAVIRF
Enzyme Length 753
Uniprot Accession Number P19835
Absorption
Active Site ACT_SITE 214; /note="Acyl-ester intermediate"; /evidence="ECO:0000255|PROSITE-ProRule:PRU10039, ECO:0000269|PubMed:1991511"; ACT_SITE 340; /note="Charge relay system"; /evidence="ECO:0000269|PubMed:1991511"; ACT_SITE 455; /note="Charge relay system"; /evidence="ECO:0000269|PubMed:1991511"
Activity Regulation ACTIVITY REGULATION: Activated by bile salts such as sodium taurocholate. {ECO:0000269|PubMed:10220579}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; Evidence={ECO:0000269|PubMed:8471055};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12045; Evidence={ECO:0000305|PubMed:8471055}; CATALYTIC ACTIVITY: Reaction=1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + di-(9Z)-octadecenoylglycerol + H(+); Xref=Rhea:RHEA:38575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:53753, ChEBI:CHEBI:75945; Evidence={ECO:0000269|PubMed:10220579};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38576; Evidence={ECO:0000305|PubMed:10220579}; CATALYTIC ACTIVITY: Reaction=1,2,3-trioctanoylglycerol + H2O = dioctanoylglycerol + H(+) + octanoate; Xref=Rhea:RHEA:47864, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25646, ChEBI:CHEBI:76978, ChEBI:CHEBI:88066; Evidence={ECO:0000269|PubMed:27650499};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47865; Evidence={ECO:0000305|PubMed:27650499}; CATALYTIC ACTIVITY: Reaction=a sterol ester + H2O = a fatty acid + a sterol + H(+); Xref=Rhea:RHEA:10100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15889, ChEBI:CHEBI:28868, ChEBI:CHEBI:35915; EC=3.1.1.13; Evidence={ECO:0000269|PubMed:8471055};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10101; Evidence={ECO:0000305|PubMed:8471055}; CATALYTIC ACTIVITY: Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, ChEBI:CHEBI:46898; Evidence={ECO:0000250|UniProtKB:P07882};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; Evidence={ECO:0000250|UniProtKB:P07882}; CATALYTIC ACTIVITY: Reaction=an acetyl ester + H2O = acetate + an aliphatic alcohol + H(+); Xref=Rhea:RHEA:12957, ChEBI:CHEBI:2571, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:47622; EC=3.1.1.6; Evidence={ECO:0000269|PubMed:10220579};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12958; Evidence={ECO:0000305|PubMed:10220579}; CATALYTIC ACTIVITY: Reaction=a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+); Xref=Rhea:RHEA:47348, ChEBI:CHEBI:2571, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:50477; Evidence={ECO:0000250|UniProtKB:P07882};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47349; Evidence={ECO:0000250|UniProtKB:P07882}; CATALYTIC ACTIVITY: Reaction=9-hexadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + H(+) + hexadecanoate; Xref=Rhea:RHEA:52052, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:83670, ChEBI:CHEBI:136286; Evidence={ECO:0000269|PubMed:27509211};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52053; Evidence={ECO:0000305|PubMed:27509211}; CATALYTIC ACTIVITY: Reaction=9-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-octadecenoate + 9-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52048, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:136282, ChEBI:CHEBI:136286; Evidence={ECO:0000269|PubMed:27509211};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52049; Evidence={ECO:0000305|PubMed:27509211}; CATALYTIC ACTIVITY: Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H(+) + hexadecanoate + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:40435, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:72998; Evidence={ECO:0000250|UniProtKB:P07882};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40436; Evidence={ECO:0000250|UniProtKB:P07882}; CATALYTIC ACTIVITY: Reaction=12-hexadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + H(+) + hexadecanoate; Xref=Rhea:RHEA:52056, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:83677, ChEBI:CHEBI:84201; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52057; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=12-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-octadecenoate + 12-hydroxyoctadecanoate + H(+); Xref=Rhea:RHEA:52060, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:84201, ChEBI:CHEBI:136302; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52061; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=13-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-octadecenoate + 13-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52064, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:136303, ChEBI:CHEBI:136304; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52065; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=9-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 9-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52068, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:136286, ChEBI:CHEBI:136309; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52069; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=12-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 12-hydroxyoctadecanoate + H(+); Xref=Rhea:RHEA:52072, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:84201, ChEBI:CHEBI:136312; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52073; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=13-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 13-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52076, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:136304, ChEBI:CHEBI:136315; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52077; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=12-octadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + H(+) + octadecanoate; Xref=Rhea:RHEA:52080, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:84201, ChEBI:CHEBI:136330; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52081; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=13-octadecanoyloxy-octadecanoate + H2O = 13-hydroxy-octadecanoate + H(+) + octadecanoate; Xref=Rhea:RHEA:52084, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:136304, ChEBI:CHEBI:136335; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52085; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=5-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 5-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52092, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:136369, ChEBI:CHEBI:136370; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52093; Evidence={ECO:0000250|UniProtKB:Q64285}; CATALYTIC ACTIVITY: Reaction=9-octadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + H(+) + octadecanoate; Xref=Rhea:RHEA:52096, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:136286, ChEBI:CHEBI:136373; Evidence={ECO:0000250|UniProtKB:Q64285};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52097; Evidence={ECO:0000250|UniProtKB:Q64285};
DNA Binding
EC Number 3.1.1.13; 3.1.1.3; 3.1.1.6
Enzyme Function FUNCTION: Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs) (PubMed:8471055, PubMed:27509211, PubMed:10220579, PubMed:27650499). Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs (By similarity). Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins (PubMed:8471055, PubMed:27509211, PubMed:10220579, PubMed:27650499). {ECO:0000250|UniProtKB:Q64285, ECO:0000269|PubMed:10220579, ECO:0000269|PubMed:27509211, ECO:0000269|PubMed:27650499, ECO:0000269|PubMed:8471055}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Alternative sequence (1); Beta strand (25); Chain (1); Compositional bias (2); Disulfide bond (2); Erroneous initiation (5); Glycosylation (11); Helix (26); Mutagenesis (1); Region (3); Repeat (17); Sequence conflict (7); Signal peptide (1); Turn (7)
Keywords 3D-structure;Alternative splicing;Diabetes mellitus;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Repeat;Secreted;Serine esterase;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21784842}.
Modified Residue
Post Translational Modification PTM: N- and O-glycosylated. {ECO:0000269|PubMed:21784842}.
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000269|PubMed:1988041
Structure 3D X-ray crystallography (7)
Cross Reference PDB 1F6W; 1JMY; 6H0T; 6H0V; 6H18; 6H19; 6H1A;
Mapped Pubmed ID 11563913; 11945176; 12110666; 12821548; 12853459; 15114370; 15841033; 16266293; 17005819; 18037996; 18544793; 18803939; 19913121; 19948975; 20628086; 21346236; 21386960; 21865348; 21988832; 22412885; 22956586; 2318975; 23395566; 23770712; 24062244; 25160620; 26498142; 27602750; 27773618; 27802312; 30315106; 30359675; 31036489; 31561066; 31622635; 31963687; 32007358; 32906201; 33079780; 33417713; 7350912; 7350913;
Motif
Gene Encoded By
Mass 79,322
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=24 uM for lipoyl-4-aminobenzoate {ECO:0000269|PubMed:8471055}; KM=15 uM for triacetin {ECO:0000269|PubMed:8471055}; Vmax=45.5 pmol/min/mg enzyme toward lipoyl-4-aminobenzoate {ECO:0000269|PubMed:8471055}; Vmax=323 pmol/min/mg enzyme toward triacetin {ECO:0000269|PubMed:8471055};
Metal Binding
Rhea ID RHEA:12044; RHEA:12045; RHEA:38575; RHEA:38576; RHEA:47864; RHEA:47865; RHEA:10100; RHEA:10101; RHEA:33875; RHEA:33876; RHEA:12957; RHEA:12958; RHEA:47348; RHEA:47349; RHEA:52052; RHEA:52053; RHEA:52048; RHEA:52049; RHEA:40435; RHEA:40436; RHEA:52056; RHEA:52057; RHEA:52060; RHEA:52061; RHEA:52064; RHEA:52065; RHEA:52068; RHEA:52069; RHEA:52072; RHEA:52073; RHEA:52076; RHEA:52077; RHEA:52080; RHEA:52081; RHEA:52084; RHEA:52085; RHEA:52092; RHEA:52093; RHEA:52096; RHEA:52097
Cross Reference Brenda 3.1.1.13;