| IED ID | IndEnz0010000013 |
| Enzyme Type ID | esterase000013 |
| Protein Name |
Polyneuridine-aldehyde esterase EC 3.1.1.78 Polyneuridine aldehyde esterase |
| Gene Name | PNAE |
| Organism | Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Gentianales Apocynaceae Rauvolfioideae Vinceae Rauvolfiinae Rauvolfia Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum) |
| Enzyme Sequence | MHSAANAKQQKHFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPLMEVMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKYNEKCPADMMLDSQFSTYGNPENPGMSMILGPQFMALKMFQNCSVEDLELAKMLTRPGSLFFQDLAKAKKFSTERYGSVKRAYIFCNEDKSFPVEFQKWFVESVGADKVKEIKEADHMGMLSQPREVCKCLLDISDS |
| Enzyme Length | 264 |
| Uniprot Accession Number | Q9SE93 |
| Absorption | |
| Active Site | ACT_SITE 87; /evidence=ECO:0000269|PubMed:12071952; ACT_SITE 216; /evidence=ECO:0000269|PubMed:12071952; ACT_SITE 244; /evidence=ECO:0000269|PubMed:12071952 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by DEPC and HgCl(2). {ECO:0000269|PubMed:12071952}. |
| Binding Site | BINDING 87; /note="16-epivellosimine; covalent"; /evidence="ECO:0000269|PubMed:19496101, ECO:0007744|PDB:3GZJ" |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + polyneuridine aldehyde = 16-epivellosimine + CO2 + methanol; Xref=Rhea:RHEA:17501, ChEBI:CHEBI:15377, ChEBI:CHEBI:16425, ChEBI:CHEBI:16526, ChEBI:CHEBI:16829, ChEBI:CHEBI:17790; EC=3.1.1.78; Evidence={ECO:0000269|PubMed:10691977, ECO:0000269|PubMed:12071952};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17502; Evidence={ECO:0000269|PubMed:10691977, ECO:0000269|PubMed:12071952}; |
| DNA Binding | |
| EC Number | 3.1.1.78 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of ajmaline. {ECO:0000269|PubMed:10691977}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Alkaloid biosynthesis; ajmaline biosynthesis. {ECO:0000305}. |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (9); Binding site (1); Chain (1); Domain (1); Helix (14); Mutagenesis (11); Propeptide (1); Turn (2) |
| Keywords | 3D-structure;Alkaloid metabolism;Direct protein sequencing;Hydrolase;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 2WFL; 2WFM; 3GZJ; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 29,655 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=36 uM for polyneuridine aldehyde {ECO:0000269|PubMed:12071952}; |
| Metal Binding | |
| Rhea ID | RHEA:17501; RHEA:17502 |
| Cross Reference Brenda |