| IED ID | IndEnz0010000020 |
| Enzyme Type ID | esterase000020 |
| Protein Name |
Lysosomal acid lipase/cholesteryl ester hydrolase Acid cholesteryl ester hydrolase LAL EC 3.1.1.13 Cholesteryl esterase Lipase A Sterol esterase |
| Gene Name | Lipa Lip1 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MQLQGLVFVFTIGILLSRVPTGTVSAVDPEVNMNVTEIIMRWGYPGEEHSVLTGDGYILSIHRIPRGRKNHFGKGPRPVVYLQHGLLADSSNWVTNIDNSSLGFLLADAGFDVWMGNSRGNTWSLKHKTLSVSQDEFWAFSFDEMAKYDLPASINYILNKTGQEQIYYVGHSQGCTIGFIAFSQMPELAKKIKMFLVLAPVLSLNFASGPLLQLGRLPDPLLKDMFGQKQFLPQSAMLKWLSIHVCTHVIMKELCANVFFLLCGFNEKNLNMSRVDVYTTHCPAGTSVQNMLHWGQVFKYRKLQAFDWGSSEKNYFHYNQSFPPSYNIKNMRLPTALWSGGRDWLADINDITILLTQIPKLVYHKNIPEWDHLDFIWGLDAPWKLYDEIISLMKKYQ |
| Enzyme Length | 397 |
| Uniprot Accession Number | Q9Z0M5 |
| Absorption | |
| Active Site | ACT_SITE 172; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 372; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a sterol ester + H2O = a fatty acid + a sterol + H(+); Xref=Rhea:RHEA:10100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15889, ChEBI:CHEBI:28868, ChEBI:CHEBI:35915; EC=3.1.1.13; Evidence={ECO:0000250|UniProtKB:P38571}; CATALYTIC ACTIVITY: Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate + cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823, ChEBI:CHEBI:46898; Evidence={ECO:0000250|UniProtKB:P38571};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876; Evidence={ECO:0000250|UniProtKB:P38571}; |
| DNA Binding | |
| EC Number | 3.1.1.13 |
| Enzyme Function | FUNCTION: Catalyzes the deacylation of triacylglyceryl and cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol. {ECO:0000250|UniProtKB:P38571}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Glycosylation (5); Propeptide (1); Sequence conflict (3); Signal peptide (1) |
| Keywords | Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Lysosome;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome {ECO:0000250|UniProtKB:Q64194}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylation is not essential for catalytic activity. {ECO:0000250|UniProtKB:P38571}. |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11290820; 11487567; 14644759; 14681479; 16127159; 16380916; 16602821; 16936266; 18039279; 19179613; 20348241; 21289032; 21308776; 21697390; 21900179; 22045912; 22383970; 22395110; 23990661; 24136225; 24287405; 24370824; 24837159; 24882582; 25000979; 25086775; 25124681; 25147230; 25450374; 25807535; 26212911; 26625314; 27354281; 27461363; 27480124; 27997605; 28402950; 28924047; 29166610; 29374543; 29599133; 30639734; 31023823; 32361002; 33113360; 33159889; 33357458; 33792344; 34416392; 34624333; 34638755; 34685599; 7292252; 9524282; 9700186; |
| Motif | |
| Gene Encoded By | |
| Mass | 45,325 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:10100; RHEA:33875; RHEA:33876 |
| Cross Reference Brenda |