| IED ID | IndEnz0010000059 |
| Enzyme Type ID | esterase000059 |
| Protein Name |
Multifunctional esterase EC 3.1.1.- Multiple nucleophilic elbowed esterase MNEE |
| Gene Name | sce3277 |
| Organism | Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56)) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria delta/epsilon subdivisions Deltaproteobacteria Myxococcales (fruiting gliding bacteria) Sorangiineae Polyangiaceae Sorangium Sorangium cellulosum (Polyangium cellulosum) Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56)) |
| Enzyme Sequence | MDQFKTHVLGLASLSLALLVALPARGASLQKVNQSEWGADGLPSYVNMYIYVPDKLATKPPIVVAPHHCQGNGQGTFSEMSSLVSIANTSGFIMIFPEATGQNCWDAGSTRSLNHGGGGDTGAIVQMVKYTLAKYGGDAGRVYSVGGSSGGIMTEALLGVYPDVFMAGVSLMGVPCGCWAEGYNDVTGTGSSAQWSGPCGGGNVTKTGQQWGDLVRSYYPGYTGHRPRLQHWHGTADTILSYKNMAEDIKEWTNVLGLSETPSGTDTPKRGTTRQFWKSACGYTVYEAFSMDGVGHAVPFDGPAVAAYFGLDRAGGQDPETAACPGAVPGGSDTGGAGGATGAGGAGGEAGTGGAGGEVGAGGAGGEAGAGAGGAGGVTVSGSGGSAGSGGAMDTASGGANGQDASAGTGDSTGGEGSSSGCSCAVGNDARDAGAQAGFLLAALGLLLGRQKRRPR |
| Enzyme Length | 456 |
| Uniprot Accession Number | A9GMG8 |
| Absorption | |
| Active Site | ACT_SITE 148; /note=Nucleophile; /evidence=ECO:0000305|PubMed:25907516; ACT_SITE 149; /note=Nucleophile; /evidence=ECO:0000305|PubMed:25907516; ACT_SITE 386; /note=Nucleophile; /evidence=ECO:0000305|PubMed:25907516; ACT_SITE 412; /note=Nucleophile; /evidence=ECO:0000305|PubMed:25907516; ACT_SITE 419; /note=Nucleophile; /evidence=ECO:0000305|PubMed:25907516 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Esterase with broad substrate specificity, that may be involved in degradation of plant biomass present in the habitat of S.cellulosum. In vitro, displays acetyl esterase activity, aryl esterase activity, paraben esterase activity, carboxyl esterase activity, poly-3-hydroxybutyric acid (PHB) depolymerase activity, and a low feruloyl esterase activity. Does not possess any paraoxonase activity. Is able to bind to multiple substrate molecules simultaneously. {ECO:0000269|PubMed:25907516}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (5); Chain (1); Compositional bias (1); Mutagenesis (5); Region (1); Signal peptide (1); Transmembrane (1) |
| Keywords | Cell membrane;Hydrolase;Membrane;Reference proteome;Serine esterase;Signal;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 45,126 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |