| IED ID | IndEnz0010000085 |
| Enzyme Type ID | esterase000085 |
| Protein Name |
Venom carboxylesterase-6 EC 3.1.1.1 allergen Api m 8 |
| Gene Name | |
| Organism | Apis mellifera (Honeybee) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Hymenoptera Apocrita (wasps ants and bees) Aculeata Apoidea (bees) Apidae (bumble bees and honey bees) Apinae (honey bees) Apini Apis Apis mellifera (Honeybee) |
| Enzyme Sequence | MYMLKLSYILLFLGFVKFSWQDKQVPKVSTFTGNIRGYYKKSRSDRLYEAYEGIPYAQSPVGKFRFQPPRPIKKWSKDLSATKKSSVCMQYLMTFTTHGNRVKGSEDCLYINIYVPVRNNRKPLLPVMFWIHGGAFQFASGNEANETLFMDRNIVFVAINYRLGPFGFLSTGDIVVPGNMGLKDQSMALRWVFNNIKSFGGNPNKITIFGMSAGGASVHYHYLSPMSAGLFKRGISISGVAFCPWAQTKHAPEKAKKLGALMKCRTDNTKKMIDCLQSRPARIIAQAVGDFMFWLYNPFTPFGPVVETYGSNPFISNSPINIINNGQVYDVPWISGVVSKEGLYTAAEFVDNAKLLWHLNDHWDEIAPYLLDFNYTIPLDQHRQVAKKIKNYYLRSGPINYDKVESIIQMMSDRLFNIDFEKAVRLQARINKSPVWTYYYSYRAEHSVSEILSGGSTTDYGVCHGDDIFLTLNSIISNVTKPQDLAMQQLLINFYTSFAIQGIPYIDEASWPSLNPNDPDFRYLHIVNFTNIKMEVNNNFANKSFWKTIPFNENKLN |
| Enzyme Length | 557 |
| Uniprot Accession Number | B2D0J5 |
| Absorption | |
| Active Site | ACT_SITE 212; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 341; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 464; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039}; |
| DNA Binding | |
| EC Number | 3.1.1.1 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (2); Glycosylation (5); Signal peptide (1) |
| Keywords | Allergen;Disulfide bond;Glycoprotein;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 63,637 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21164 |
| Cross Reference Brenda |