| IED ID | IndEnz0010000099 |
| Enzyme Type ID | esterase000099 |
| Protein Name |
Esterase E4 EC 3.1.1.1 Carboxylic-ester hydrolase |
| Gene Name | |
| Organism | Myzus persicae (Green peach aphid) (Aphis persicae) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Paraneoptera Hemiptera Sternorrhyncha Aphidomorpha (aphids) Aphidoidea Aphididae (aphids) Aphidinae Macrosiphini Myzus Myzus persicae (Green peach aphid) (Aphis persicae) |
| Enzyme Sequence | MKNTCGILLNLFLFIGCFLTCSASNTPKVQVHSGEIAGGFEYTYNGRKIYSFLGIPYASPPVQNNRFKEPQPVQPWLGVWNATVPGSACLGIEFGSGSKIIGQEDCLFLNVYTPKLPQENSAGDLMNVIVHIHGGGYYFGEGILYGPHYLLDNNDFVYVSINYRLGVLGFASTGDGVLTGNNGLKDQVAALKWIQQNIVAFGGDPNSVTITGMSAGASSVHNHLISPMSKGLFNRAIIQSGSAFCHWSTAENVAQKTKYIANLMGCPTNNSVEIVECLRSRPAKAIAKSYLNFMPWRNFPFTPFGPTVEVAGYEKFLPDIPEKLVPHDIPVLISIAQDEGLIFSTFLGLENGFNELNNNWNEHLPHILDYNYTISNENLRFKTAQDIKEFYFGDKPISKETKSNLSKMISDRSFGYGTSKAAQHIAAKNTAPVYFYEFGYSGNYSYVAFFDPKSYSRGSSPTHGDETSYVLKMDGFYVYDNEEDRKMIKTMVNIWATFIKSGVPDTENSEIWLPVSKNLADPFRFTKITQQQTFEAREQSTTGIMNFGVAYH |
| Enzyme Length | 552 |
| Uniprot Accession Number | P35501 |
| Absorption | |
| Active Site | ACT_SITE 214; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 339; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 463; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10039}; |
| DNA Binding | |
| EC Number | 3.1.1.1 |
| Enzyme Function | FUNCTION: Overproduction of nonspecific esterases is a common mechanism of resistance to organophosphate insecticides. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (2); Glycosylation (5); Signal peptide (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000269|PubMed:8373371 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 61,348 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21164 |
| Cross Reference Brenda |