| IED ID | IndEnz0010000120 |
| Enzyme Type ID | esterase000120 |
| Protein Name |
Acetylcholinesterase AChE EC 3.1.1.7 |
| Gene Name | |
| Organism | Leptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Coleoptera Polyphaga Cucujiformia Chrysomeloidea Chrysomelidae (leaf beetles) Chrysomelinae Doryphorini Leptinotarsa Leptinotarsa decemlineata (Colorado potato beetle) (Doryphora decemlineata) |
| Enzyme Sequence | MGQLSILCLFVTVCASVCGYSWPSDETTTKPSQFKDFHTDPLVVETTSGLVRGYSKTVLGREVHVFTGIPFAKPPIEQLRFKKPVPIDPWHGILDATKQPNSCFQERYEYFPGFEGEEMWNPNTNISEDCLYLNIWVPQRLRIRHHADKPTIDRPKVPVLIWIYGGGYMSGTATLDVYDADIIAATSDVIVASMQYRLGSFGFLYLNRYFPRGSDETPGNMGLWDQILAIRWIKDNAAAFGGDPDLITLFGESAGGGSISIHLISPVTKGLVRRGIMQSGTMNAPWSYMSGERAEQIGKILIQDCGCNVSLLENSPRKVMDCMRAVDAKTISLQQWNSYSGILGFPSTPTIEGVLLPKHPMDMLAEGDYEDMEILLGSNHDEGTYFLLYDFIDFFEKDGPSFLQREKYHDIIDTIFKNMSRLERDAIVFQYTNWEHVHDGYLNQKMIGDVVGDYFFVCPTNNFAEVAADRGMKVFYYYFTHRTSTSLWGEWMGVIHGDEVEYVFGHPLNMSLQFNSRERELSLKIMQAFARFATTGKPVTDDVNWPLYTKDQPQYFIFNADKNGIGKGPRATACAFWNDFLPKLRDNSGSEEAPCVNTYLSKIRSSSNELLPPSTSLVLIWIMTLLNAL |
| Enzyme Length | 629 |
| Uniprot Accession Number | Q27677 |
| Absorption | |
| Active Site | ACT_SITE 253; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 382; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 496; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetylcholine + H2O = acetate + choline + H(+); Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7; |
| DNA Binding | |
| EC Number | 3.1.1.7 |
| Enzyme Function | FUNCTION: Rapidly hydrolyzes choline released into the synapse. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Glycosylation (4); Lipidation (1); Propeptide (1); Signal peptide (1) |
| Keywords | Cell junction;Cell membrane;Disulfide bond;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Membrane;Neurotransmitter degradation;Serine esterase;Signal;Synapse |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell junction, synapse {ECO:0000250}. Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}. Note=Attached to the membrane of the neuronal cholinergic synapses by a GPI-anchor. {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: The N-terminus is blocked. |
| Signal Peptide | SIGNAL 1..38; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 71,142 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:17561 |
| Cross Reference Brenda |