Detail Information for IndEnz0010000121
IED ID IndEnz0010000121
Enzyme Type ID esterase000121
Protein Name Acetylcholinesterase-1
AChE
EC 3.1.1.7
Gene Name
Organism Trittame loki (Brush-footed trapdoor spider)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Chelicerata Arachnida Araneae (spiders) Mygalomorphae (mygalomorph spiders) Barychelidae Trittame Trittame loki (Brush-footed trapdoor spider)
Enzyme Sequence MMLPRCFVTVLLMSSVLYIGGETSSEVLGPVVSTEAGSFQGMELTTHGERVIYAFLGIPYAKPPTGLLRFKKPQPADFIQGTYKATEKPPSCFQLDSDLQLPWADPDSPMKEDCLFLNLWTPASLSTEEEELKSVMVWIHGGGYTSGSSALDVYDGQTLSSSGDVVVVTMNYRLDAFGFLNSLTEDAPGNMALYDQLLALQWVHTNIKYFGGDPNKVTLFGESVGAFATSFLALSPLTKGLFQKIMLESGSAYNKLTVNSIDQAKNNNQLATLVGCANETFTLISNPEEVVACMREVAPAKFTQTYYKELGSEREKINFIFWPHFGDDILPTRTAELIKEKNLTALFAGVNSVEGSALSVFFFPEVYQMFVESNLTLTKAYATILMNEFFKVFNFQDSAKAIEFYLGDVEDDDEEGIRSALFGVVGDYIITCPTIYLADKYSERGANVQFYRFDRRPSTSQWPPEWMGAAHNDEIQFVFGMPVRYPEKYTEEERTLSEYMTRTWTNFVKSEDLKLKNGSQWPSYSLSEPQFATLQTNEQIIGSGQRKAECDFWRPYFDI
Enzyme Length 559
Uniprot Accession Number W4VSJ0
Absorption
Active Site ACT_SITE 223; /note=Acyl-ester intermediate; /evidence=ECO:0000250; ACT_SITE 354; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 471; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=acetylcholine + H2O = acetate + choline + H(+); Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7;
DNA Binding
EC Number 3.1.1.7
Enzyme Function FUNCTION: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (3); Glycosylation (3); Modified residue (1); Region (1); Signal peptide (1)
Keywords Disulfide bond;Glycoprotein;Hydrolase;Neurotransmitter degradation;Phosphoprotein;Secreted;Serine esterase;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue MOD_RES 223; /note=Phosphoserine; /evidence=ECO:0000250
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 62,782
Kinetics
Metal Binding
Rhea ID RHEA:17561
Cross Reference Brenda