| IED ID | IndEnz0010000122 |
| Enzyme Type ID | esterase000122 |
| Protein Name |
Acetylcholinesterase AChE EC 3.1.1.7 |
| Gene Name | Ache |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MRPPWYPLHTPSLAFPLLFLLLSLLGGGARAEGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL |
| Enzyme Length | 614 |
| Uniprot Accession Number | P21836 |
| Absorption | |
| Active Site | ACT_SITE 234; /note=Acyl-ester intermediate; ACT_SITE 365; /note=Charge relay system; ACT_SITE 478; /note=Charge relay system |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetylcholine + H2O = acetate + choline + H(+); Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7; |
| DNA Binding | |
| EC Number | 3.1.1.7 |
| Enzyme Function | FUNCTION: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (24); Chain (1); Disulfide bond (4); Glycosylation (3); Helix (28); Signal peptide (1); Turn (7) |
| Keywords | 3D-structure;Alternative splicing;Cell junction;Cell membrane;Disulfide bond;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Membrane;Neurotransmitter degradation;Reference proteome;Secreted;Serine esterase;Signal;Synapse |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell junction, synapse. Secreted. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.; SUBCELLULAR LOCATION: [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31 |
| Structure 3D | X-ray crystallography (87) |
| Cross Reference PDB | 1C2B; 1C2O; 1J06; 1J07; 1KU6; 1MAA; 1MAH; 1N5M; 1N5R; 1Q83; 1Q84; 2C0P; 2C0Q; 2GYU; 2GYV; 2GYW; 2H9Y; 2HA0; 2HA2; 2HA3; 2HA4; 2HA5; 2HA6; 2HA7; 2JEY; 2JEZ; 2JF0; 2JGE; 2JGF; 2JGI; 2JGJ; 2JGK; 2JGL; 2JGM; 2WHP; 2WHQ; 2WHR; 2WLS; 2WU3; 2WU4; 2XUD; 2XUF; 2XUG; 2XUH; 2XUI; 2XUJ; 2XUK; 2XUO; 2XUP; 2XUQ; 2Y2U; 2Y2V; 3DL4; 3DL7; 3ZLT; 3ZLU; 3ZLV; 4A16; 4A23; 4ARA; 4ARB; 4B7Z; 4B80; 4B81; 4B82; 4B83; 4B84; 4B85; 4BC0; 4BC1; 4BTL; 5DTI; 5DTJ; 5EHN; 5EHQ; 5EHZ; 5EIA; 5EIE; 5EIH; 5FKJ; 5FPP; 5FUM; 5HCU; 5OV9; 6FSD; 6FSE; 6TD2; |
| Mapped Pubmed ID | 10069332; 10357937; 10409660; 10421464; 10483521; 10521398; 10521413; 10591541; 10657302; 10707974; 10764649; 10794165; 10869390; 10890884; 10920239; 10936216; 10981960; 10995443; 11018052; 11301191; 11312300; 11375492; 11511515; 11602663; 11749040; 11799248; 11929041; 11934471; 11943824; 12065653; 12128253; 12140295; 12196517; 12351730; 12441053; 12466851; 12509514; 12533614; 12571103; 12571110; 12665427; 12667913; 12682014; 12759360; 12799142; 12895513; 1358806; 1400357; 14511322; 14535959; 14581933; 14622273; 14645660; 14681479; 14757816; 15123727; 15170228; 15207914; 15345536; 15454088; 15474030; 15497153; 15541311; 15579149; 15666354; 15851520; 15878846; 15923401; 15978004; 16141072; 16259971; 16274683; 16289062; 16365392; 16380450; 16388582; 16429497; 16429555; 16429580; 16434405; 16467531; 16602821; 16615898; 16691566; 16766072; 16837465; 16876764; 16904653; 16987237; 17038428; 17050626; 17131407; 17192635; 17192637; 17192642; 17192646; 17194517; 17212694; 17216607; 17241270; 17272501; 17324938; 17402711; 17420226; 1744105; 17443135; 17476278; 17538015; 17916349; 17942321; 17948252; 17960645; 17963737; 18023504; 18160706; 18167304; 18194455; 18243359; 18299393; 18313329; 18322091; 18367596; 18384970; 18511416; 18560895; 18647752; 18785927; 18975951; 18977317; 19061871; 19357277; 19536291; 19554387; 19605412; 19621438; 19645739; 19705444; 19714254; 19732756; 19766675; 20005135; 20053883; 20054652; 20107080; 20153304; 20178819; 20381477; 20805581; 20883790; 20919754; 21090615; 21267068; 21538119; 21621296; 21723318; 21865157; 21870149; 21978551; 22052791; 22098391; 22140425; 22236578; 22246100; 22266424; 22348892; 22537499; 22570738; 22627288; 22739110; 22747514; 22832508; 22988430; 22992950; 23047022; 23123627; 23147415; 23161758; 23201480; 23219600; 23236169; 23339663; 23341629; 23376121; 23401542; 23440963; 23897820; 23984975; 24312449; 24952961; 25113559; 25131634; 25135772; 25269881; 25683697; 25853328; 26036470; 26079645; 26350723; 26506622; 26731630; 26929400; 27021952; 27140636; 27316341; 27317840; 27378627; 27598521; 27737765; 27807596; 28118357; 28427893; 28867801; 28973166; 29263397; 29323161; 29688375; 30076217; 30339371; 30816118; 32290105; 32610016; 32708917; 32898154; 33235206; 33574433; 34091985; 7512968; 7582132; 7605630; 7605908; 7806243; 7829523; 7885444; 8010411; 8429032; 8488842; 8566217; 8626792; 8651510; 8653788; 8660874; 8666981; 8794865; 8824813; 8994044; 9223334; 9240560; 9256502; 9376320; 9401056; 9676744; 9679061; 9789818; |
| Motif | |
| Gene Encoded By | |
| Mass | 68,169 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:17561 |
| Cross Reference Brenda | 3.1.1.7; |