| IED ID | IndEnz0010000123 |
| Enzyme Type ID | esterase000123 |
| Protein Name |
Acetylcholinesterase AChE EC 3.1.1.7 |
| Gene Name | ache |
| Organism | Tetronarce californica (Pacific electric ray) (Torpedo californica) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Chondrichthyes Elasmobranchii (elasmobranchs) Batoidea (rays) Torpediniformes Torpedinidae (electric rays) Tetronarce Tetronarce californica (Pacific electric ray) (Torpedo californica) |
| Enzyme Sequence | MNLLVTSSLGVLLHLVVLCQADDHSELLVNTKSGKVMGTRVPVLSSHISAFLGIPFAEPPVGNMRFRRPEPKKPWSGVWNASTYPNNCQQYVDEQFPGFSGSEMWNPNREMSEDCLYLNIWVPSPRPKSTTVMVWIYGGGFYSGSSTLDVYNGKYLAYTEEVVLVSLSYRVGAFGFLALHGSQEAPGNVGLLDQRMALQWVHDNIQFFGGDPKTVTIFGESAGGASVGMHILSPGSRDLFRRAILQSGSPNCPWASVSVAEGRRRAVELGRNLNCNLNSDEELIHCLREKKPQELIDVEWNVLPFDSIFRFSFVPVIDGEFFPTSLESMLNSGNFKKTQILLGVNKDEGSFFLLYGAPGFSKDSESKISREDFMSGVKLSVPHANDLGLDAVTLQYTDWMDDNNGIKNRDGLDDIVGDHNVICPLMHFVNKYTKFGNGTYLYFFNHRASNLVWPEWMGVIHGYEIEFVFGLPLVKELNYTAEEEALSRRIMHYWATFAKTGNPNEPHSQESKWPLFTTKEQKFIDLNTEPMKVHQRLRVQMCVFWNQFLPKLLNATACDGELSSSGTSSSKGIIFYVLFSILYLIF |
| Enzyme Length | 586 |
| Uniprot Accession Number | P04058 |
| Absorption | |
| Active Site | ACT_SITE 221; /note=Acyl-ester intermediate; ACT_SITE 348; /note=Charge relay system; ACT_SITE 461; /note=Charge relay system |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by substrate concentrations above 0.5 mM. {ECO:0000269|PubMed:16763558}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetylcholine + H2O = acetate + choline + H(+); Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7; |
| DNA Binding | |
| EC Number | 3.1.1.7 |
| Enzyme Function | FUNCTION: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Beta strand (26); Chain (1); Disulfide bond (4); Glycosylation (4); Helix (27); Lipidation (1); Mutagenesis (6); Propeptide (1); Signal peptide (1); Turn (5) |
| Keywords | 3D-structure;Alternative splicing;Cell junction;Cell membrane;Direct protein sequencing;Disulfide bond;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Membrane;Neurotransmitter degradation;Serine esterase;Signal;Synapse |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.; SUBCELLULAR LOCATION: [Isoform T]: Cell membrane; Peripheral membrane protein. Cell junction, synapse. Note=Attached to the membrane through disulfide linkage with the collagenic subunit, itself bound to the membrane. |
| Modified Residue | |
| Post Translational Modification | PTM: An interchain disulfide bond is present in what becomes position 593 of the T isoform. |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000269|PubMed:3900071 |
| Structure 3D | X-ray crystallography (123) |
| Cross Reference PDB | 1ACJ; 1ACL; 1AMN; 1AX9; 1CFJ; 1DX6; 1E3Q; 1E66; 1EA5; 1EEA; 1EVE; 1FSS; 1GPK; 1GPN; 1GQR; 1GQS; 1H22; 1H23; 1HBJ; 1JJB; 1OCE; 1ODC; 1QID; 1QIE; 1QIF; 1QIG; 1QIH; 1QII; 1QIJ; 1QIK; 1QIM; 1QTI; 1SOM; 1U65; 1UT6; 1VOT; 1VXO; 1VXR; 1W4L; 1W6R; 1W75; 1W76; 1ZGB; 1ZGC; 2ACE; 2ACK; 2BAG; 2C4H; 2C58; 2C5F; 2C5G; 2CEK; 2CKM; 2CMF; 2DFP; 2J3D; 2J3Q; 2J4F; 2V96; 2V97; 2V98; 2VA9; 2VJA; 2VJB; 2VJC; 2VJD; 2VQ6; 2VT6; 2VT7; 2W6C; 2WFZ; 2WG0; 2WG1; 2WG2; 2XI4; 3GEL; 3I6M; 3I6Z; 3M3D; 3ZV7; 4TVK; 4W63; 4X3C; 5BWB; 5BWC; 5DLP; 5E2I; 5E4J; 5E4T; 5EHX; 5EI5; 5IH7; 5NAP; 5NAU; 5NUU; 6EUC; 6EUE; 6EWK; 6EZG; 6EZH; 6FLD; 6FQN; 6G17; 6G1U; 6G1V; 6G1W; 6G4M; 6G4N; 6G4O; 6G4P; 6H12; 6H13; 6H14; 6TT0; 7AIS; 7AIT; 7AIU; 7AIV; 7AIW; 7AIX; 7B2W; 7B38; 7B8E; |
| Mapped Pubmed ID | 10089512; 10476864; 10514292; 10639129; 10934357; 11119642; 11449566; 11563919; 12095250; 12140604; 12196020; 12351819; 12517147; 15563167; 1575745; 16076210; 16594661; 16711737; 16913695; 16942022; 18007027; 18512913; 1863425; 18701720; 19326912; 21594947; 22390827; 25259726; 26327369; 26632651; 26990888; 2850366; 29534488; 29651884; 29672968; 30125110; 30447434; 30798053; 30851693; 32435398; 3322595; 33686648; 3384821; 7738603; 7981200; 8003956; 9278281; 9428657; 9839013; |
| Motif | |
| Gene Encoded By | |
| Mass | 65,906 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:17561 |
| Cross Reference Brenda | 3.1.1.7; |