| IED ID | IndEnz0010000127 |
| Enzyme Type ID | esterase000127 |
| Protein Name |
Acetylajmalan esterase EC 3.1.1.80 |
| Gene Name | AAE |
| Organism | Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Gentianales Apocynaceae Rauvolfioideae Vinceae Rauvolfiinae Rauvolfia Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum) |
| Enzyme Sequence | MGFARLLHLVFSLLVFAGITNGLICPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGTPTGRCSDGRLIIDFIATALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDIHSHLSAQLNWFRTYLGSICSTPKECSNKLKNALFILGNIGNNDVNYAFPNRTIEEIRAYVPFITEAVANATREIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSSLNNLSIYFNSLFQRALASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCGIGGPYNYDPDRECGSRGVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKALKCSYSNIQPFLREGEGRQALRLNERE |
| Enzyme Length | 387 |
| Uniprot Accession Number | Q3MKY2 |
| Absorption | |
| Active Site | ACT_SITE 36; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q00017; ACT_SITE 337; /evidence=ECO:0000250|UniProtKB:Q00017; ACT_SITE 340; /evidence=ECO:0000250|UniProtKB:Q00017 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=17-O-acetylajmaline + H2O = acetate + ajmaline + H(+); Xref=Rhea:RHEA:22124, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:58567, ChEBI:CHEBI:58679; EC=3.1.1.80; Evidence={ECO:0000269|PubMed:16133216}; CATALYTIC ACTIVITY: Reaction=17-O-acetylnorajmaline + H2O = acetate + H(+) + norajmaline; Xref=Rhea:RHEA:23796, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:77618, ChEBI:CHEBI:77725; EC=3.1.1.80; Evidence={ECO:0000269|PubMed:16133216}; |
| DNA Binding | |
| EC Number | 3.1.1.80 |
| Enzyme Function | FUNCTION: Deacetylates 17-O-acetylajmaline and 17-O-acetylnorajmaline, but is inactive toward other acetylated alkaloids. {ECO:0000269|PubMed:16133216}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 53 degrees Celsius. {ECO:0000269|PubMed:16133216}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.0. Half maximum activity is seen at pH 6.5 and 9.8. {ECO:0000269|PubMed:16133216}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (5); Signal peptide (1) |
| Keywords | Alkaloid metabolism;Glycoprotein;Hydrolase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 42,729 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.7 mM for 17-O-acetylnorajmaline {ECO:0000269|PubMed:16133216}; KM=0.12 mM for 17-O-acetylajmaline {ECO:0000269|PubMed:16133216}; |
| Metal Binding | |
| Rhea ID | RHEA:22124; RHEA:23796 |
| Cross Reference Brenda | 3.1.1.80; |