Detail Information for IndEnz0010000133
IED ID IndEnz0010000133
Enzyme Type ID esterase000133
Protein Name Diacylglycerol acyltransferase/mycolyltransferase Ag85A
DGAT
EC 2.3.1.122
EC 2.3.1.20
Acyl-CoA:diacylglycerol acyltransferase
Antigen 85 complex A
85A
Ag85A
Fibronectin-binding protein A
Fbps A
Fragment
Gene Name fbpA
Organism Mycobacterium marinum
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium marinum
Enzyme Sequence PTGSGVVGLSMAGSSALILAAYHPDQFVYSGSLSALLDPSQGMGPSLIGLAMGDAGGYKASDMWGPKDDPAWARNDPMLQVGKLVANNTRIWVYCGNGKPSDLGGDNLPAKFLEGFVRTSNMKFQAAYNAAGGHNAVWN
Enzyme Length 139
Uniprot Accession Number Q9KH57
Absorption
Active Site ACT_SITE 10; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 114; /evidence=ECO:0000250
Activity Regulation
Binding Site BINDING 10; /note=Substrate; /evidence=ECO:0000250; BINDING 38; /note=Substrate; /evidence=ECO:0000250; BINDING 123; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287, ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20; CATALYTIC ACTIVITY: Reaction=2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose 6,6'-bismycolate + alpha,alpha-trehalose; Xref=Rhea:RHEA:23472, ChEBI:CHEBI:16551, ChEBI:CHEBI:18195, ChEBI:CHEBI:18234; EC=2.3.1.122;
DNA Binding
EC Number 2.3.1.122; 2.3.1.20
Enzyme Function FUNCTION: The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Binding site (3); Chain (1); Non-terminal residue (2); Region (1)
Keywords Acyltransferase;Cell wall;Cytoplasm;Secreted;Transferase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall. Cytoplasm.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 14,449
Kinetics
Metal Binding
Rhea ID RHEA:10868; RHEA:23472
Cross Reference Brenda