IED ID | IndEnz0010000156 |
Enzyme Type ID | esterase000156 |
Protein Name |
Pimeloyl- acyl-carrier protein methyl ester esterase EC 3.1.1.85 Biotin synthesis protein BioH Carboxylesterase BioH |
Gene Name | bioH VC_2718 |
Organism | Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) |
Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Vibrionales Vibrionaceae Vibrio Vibrio cholerae Vibrio cholerae O1 Vibrio cholerae O1 biovar El Tor Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) |
Enzyme Sequence | MTMALYWQVSGQGQDLVLVHGWGMNGAVWQQTAQALSDHFRVHVVDLPGYGHSAEQHAASLEEIAQALLEHAPRNAIWVGWSLGGLVATHMALHHSDYVSKLVTVASSPKFAAQGSWRGIQPDVLTAFTDQLVADFQLTIERFMALQAMGSPSARQDVKVLKQAVLSRPMPNPQSLLAGLTMLAEVDLRDELQHISVPMLRLYGRLDGLVPAKVARDLNHLAPYSEAFMFDQSSHAPFMTEAEAFCQQLIEFATR |
Enzyme Length | 255 |
Uniprot Accession Number | Q9KNL4 |
Absorption | |
Active Site | ACT_SITE 82; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 207; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 235; /evidence=ECO:0000255|HAMAP-Rule:MF_01260 |
Activity Regulation | |
Binding Site | BINDING 22; /note=Substrate; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; BINDING 235; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_01260 |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6-carboxyhexanoyl-[ACP] + H(+) + methanol; Xref=Rhea:RHEA:42700, Rhea:RHEA-COMP:9955, Rhea:RHEA-COMP:10186, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:78846, ChEBI:CHEBI:82735; EC=3.1.1.85; Evidence={ECO:0000255|HAMAP-Rule:MF_01260}; |
DNA Binding | |
EC Number | 3.1.1.85 |
Enzyme Function | FUNCTION: The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters. {ECO:0000255|HAMAP-Rule:MF_01260}. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Cofactor biosynthesis; biotin biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01260}. |
nucleotide Binding | |
Features | Active site (3); Binding site (2); Chain (1); Domain (1); Erroneous initiation (1); Region (2) |
Keywords | Biotin biosynthesis;Cytoplasm;Hydrolase;Reference proteome;Serine esterase |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01260}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 28,109 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:42700 |
Cross Reference Brenda |