| IED ID | IndEnz0010000168 |
| Enzyme Type ID | esterase000168 |
| Protein Name |
Acyl-protein thioesterase 1 EC 3.1.2.- Palmitoyl-protein hydrolase EC 3.1.2.22 |
| Gene Name | AN8748 |
| Organism | Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Enzyme Sequence | MSRAPFIVPALKKHTATVIMAHGLGDSGAGWVSLAHNWRRRGLFEEVTFIFPNAPMIPITVNFGMSMPGWYDITKLGRDLDFQEAVKNQDEAGILKSRDYFNSLIKEQMDQGIKPSRIVLGGFSQGGAMSLFSGITGQEKLGGVFGLSCYMLLSDRIKNYIPENFPNKKTPFFLAHGTEDDIVPHEFGKRSAEMAKELGLEDVTFNSYKYLSHSADPVEIEDLEKFLDRVIPAENGGSL |
| Enzyme Length | 239 |
| Uniprot Accession Number | Q5ASI2 |
| Absorption | |
| Active Site | ACT_SITE 124; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 180; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 213; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + S-hexadecanoyl-L-cysteinyl-[protein] = H(+) + hexadecanoate + L-cysteinyl-[protein]; Xref=Rhea:RHEA:19233, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, ChEBI:CHEBI:74151; EC=3.1.2.22; Evidence={ECO:0000250|UniProtKB:Q12354}; |
| DNA Binding | |
| EC Number | 3.1.2.-; 3.1.2.22 |
| Enzyme Function | FUNCTION: Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo. {ECO:0000250|UniProtKB:Q12354}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1) |
| Keywords | Cytoplasm;Fatty acid metabolism;Hydrolase;Lipid metabolism;Nucleus;Reference proteome;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q12354}. Nucleus {ECO:0000250|UniProtKB:Q12354}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 26,553 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:19233 |
| Cross Reference Brenda |