| IED ID | IndEnz0010000176 |
| Enzyme Type ID | esterase000176 |
| Protein Name |
Chitin deacetylase 1 BmCDA1 EC 3.5.1.41 |
| Gene Name | CDA1 |
| Organism | Bombyx mori (Silk moth) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Amphiesmenoptera Lepidoptera (butterflies and moths) Glossata Neolepidoptera Heteroneura Ditrysia Obtectomera Bombycoidea (hawk-moths) Bombycidae (silkworm moths) Bombycinae Bombyx Bombyx mori (Silk moth) |
| Enzyme Sequence | MARYARVATLAACLLFACALADGHRWRRQADETAKKDESLEQELCKDKDAGEWFRLVAGEGDNCRDVIQCTASGIQAIRCPAGLFFDIEKQTCDWKDAVKNCKLKNKERKIKPLLYTEEPLCQDGFLACGDSTCIERGLFCNGEKDCGDGSDENSCDIDNDPNRAPPCDSSQCVLPDCFCSEDGTVIPGDLPARDVPQMITITFDDAINNNNIELYKEIFNGKRKNPNGCDIKATYFVSHKYTNYSAVQETHRKGHEIAVHSITHNDDERFWSNATVDDWGKEMAGMRVIIEKFSNITDNSVVGVRAPYLRVGGNNQFTMMEEQAFLYDSTITAPLSNPRLCPYTMYFRMPHRCHGNLQSCPTRSHAVWEMVMNELDRREDPSNDEYLPGCAMVDSCSNILTGDQFYNFLNHNFDRHYEQNRAPLGLYFHAAWLKNNPEFLEAFLYWIDEILQSHNDVYFVTMTQVIQWVQNPRTVTEAKNFEPWREKCSVEGNPACWVPHSCKLTSKEVPGETINLQTCLRCPVNYPWLNDPTGDGHY |
| Enzyme Length | 539 |
| Uniprot Accession Number | H9J9M0 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Binding to the accessory protein CPAP3-A1 is essential for chitinase activity. {ECO:0000269|PubMed:30755482}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-N-acetyl-beta-D-glucosaminyl](n) + n H2O = n acetate + chitosan; Xref=Rhea:RHEA:10464, Rhea:RHEA-COMP:9593, Rhea:RHEA-COMP:9597, ChEBI:CHEBI:15377, ChEBI:CHEBI:17029, ChEBI:CHEBI:30089, ChEBI:CHEBI:57704; EC=3.5.1.41; Evidence={ECO:0000269|PubMed:30755482}; |
| DNA Binding | |
| EC Number | 3.5.1.41 |
| Enzyme Function | FUNCTION: Hydrolyzes the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin. {ECO:0000269|PubMed:30755482}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (10); Chain (1); Disulfide bond (11); Domain (2); Glycosylation (2); Helix (15); Metal binding (3); Mutagenesis (1); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Carbohydrate metabolism;Chitin degradation;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Polysaccharide degradation;Reference proteome;Secreted;Signal;Zinc |
| Interact With | |
| Induction | INDUCTION: Up-regulated in response to 20-hydroxyecdysone (20E) and juvenile hormone analog. Also up-regulated in response to the transcription factors BRC-Z2 and POUM2. {ECO:0000269|PubMed:30987273}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 5ZNS; 5ZNT; |
| Mapped Pubmed ID | 29251378; |
| Motif | |
| Gene Encoded By | |
| Mass | 61,429 |
| Kinetics | |
| Metal Binding | METAL 206; /note="Zinc"; /evidence="ECO:0000269|PubMed:30755482, ECO:0007744|PDB:5ZNT"; METAL 261; /note="Zinc; via tele nitrogen"; /evidence="ECO:0000269|PubMed:30755482, ECO:0007744|PDB:5ZNS, ECO:0007744|PDB:5ZNT"; METAL 265; /note="Zinc; via tele nitrogen"; /evidence="ECO:0000269|PubMed:30755482, ECO:0007744|PDB:5ZNS, ECO:0007744|PDB:5ZNT" |
| Rhea ID | RHEA:10464 |
| Cross Reference Brenda |