Detail Information for IndEnz0010000176
IED ID IndEnz0010000176
Enzyme Type ID esterase000176
Protein Name Chitin deacetylase 1
BmCDA1
EC 3.5.1.41
Gene Name CDA1
Organism Bombyx mori (Silk moth)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Amphiesmenoptera Lepidoptera (butterflies and moths) Glossata Neolepidoptera Heteroneura Ditrysia Obtectomera Bombycoidea (hawk-moths) Bombycidae (silkworm moths) Bombycinae Bombyx Bombyx mori (Silk moth)
Enzyme Sequence MARYARVATLAACLLFACALADGHRWRRQADETAKKDESLEQELCKDKDAGEWFRLVAGEGDNCRDVIQCTASGIQAIRCPAGLFFDIEKQTCDWKDAVKNCKLKNKERKIKPLLYTEEPLCQDGFLACGDSTCIERGLFCNGEKDCGDGSDENSCDIDNDPNRAPPCDSSQCVLPDCFCSEDGTVIPGDLPARDVPQMITITFDDAINNNNIELYKEIFNGKRKNPNGCDIKATYFVSHKYTNYSAVQETHRKGHEIAVHSITHNDDERFWSNATVDDWGKEMAGMRVIIEKFSNITDNSVVGVRAPYLRVGGNNQFTMMEEQAFLYDSTITAPLSNPRLCPYTMYFRMPHRCHGNLQSCPTRSHAVWEMVMNELDRREDPSNDEYLPGCAMVDSCSNILTGDQFYNFLNHNFDRHYEQNRAPLGLYFHAAWLKNNPEFLEAFLYWIDEILQSHNDVYFVTMTQVIQWVQNPRTVTEAKNFEPWREKCSVEGNPACWVPHSCKLTSKEVPGETINLQTCLRCPVNYPWLNDPTGDGHY
Enzyme Length 539
Uniprot Accession Number H9J9M0
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Binding to the accessory protein CPAP3-A1 is essential for chitinase activity. {ECO:0000269|PubMed:30755482}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-N-acetyl-beta-D-glucosaminyl](n) + n H2O = n acetate + chitosan; Xref=Rhea:RHEA:10464, Rhea:RHEA-COMP:9593, Rhea:RHEA-COMP:9597, ChEBI:CHEBI:15377, ChEBI:CHEBI:17029, ChEBI:CHEBI:30089, ChEBI:CHEBI:57704; EC=3.5.1.41; Evidence={ECO:0000269|PubMed:30755482};
DNA Binding
EC Number 3.5.1.41
Enzyme Function FUNCTION: Hydrolyzes the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin. {ECO:0000269|PubMed:30755482}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (10); Chain (1); Disulfide bond (11); Domain (2); Glycosylation (2); Helix (15); Metal binding (3); Mutagenesis (1); Signal peptide (1); Turn (6)
Keywords 3D-structure;Carbohydrate metabolism;Chitin degradation;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Polysaccharide degradation;Reference proteome;Secreted;Signal;Zinc
Interact With
Induction INDUCTION: Up-regulated in response to 20-hydroxyecdysone (20E) and juvenile hormone analog. Also up-regulated in response to the transcription factors BRC-Z2 and POUM2. {ECO:0000269|PubMed:30987273}.
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D X-ray crystallography (2)
Cross Reference PDB 5ZNS; 5ZNT;
Mapped Pubmed ID 29251378;
Motif
Gene Encoded By
Mass 61,429
Kinetics
Metal Binding METAL 206; /note="Zinc"; /evidence="ECO:0000269|PubMed:30755482, ECO:0007744|PDB:5ZNT"; METAL 261; /note="Zinc; via tele nitrogen"; /evidence="ECO:0000269|PubMed:30755482, ECO:0007744|PDB:5ZNS, ECO:0007744|PDB:5ZNT"; METAL 265; /note="Zinc; via tele nitrogen"; /evidence="ECO:0000269|PubMed:30755482, ECO:0007744|PDB:5ZNS, ECO:0007744|PDB:5ZNT"
Rhea ID RHEA:10464
Cross Reference Brenda