Detail Information for IndEnz0010000178
IED ID IndEnz0010000178
Enzyme Type ID esterase000178
Protein Name Asperlin biosynthesis cluster protein I
Gene Name alnI ANIA_11200
Organism Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Nidulantes Emericella nidulans (Aspergillus nidulans) Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Enzyme Sequence MFSSTRRAEGPCATELTQVSSLLPPRGPYEFSLLPTLTRPLEDLSKCIEGARQTSATANGYSPTGLVPLADSILEICQAACTAYGLVDGAIAAGVGTGSSDNSPTATGIGAAGLTGDRPSSSGASTWRCVKTPMTLGSLTLQNEEESLLARQIVYAVLTSLSALLREVYVREKDVVSETDVVGEGGVGAGAALYGREGAGAVSQCLSRVLALLGKIVPE
Enzyme Length 219
Uniprot Accession Number C8VJQ9
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Part of the gene cluster that mediates the biosynthesis of asperlin, a polyketide showing anti-inflammatory, antitumor and antibiotic activities (PubMed:30339758). The first step of the asperlin biosynthesis is the production of the intermediate 2,4,6-octatrienoic acid by the highly redusing polyketide synthase alnA with cleavage of the PKS product by the esterase alnB (PubMed:30339758). 2,4,6-octatrienoic acid is further converted to asperlin via several steps involving the remaining enzymes from the cluster (PubMed:30339758). {ECO:0000269|PubMed:30339758}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Polyketide biosynthesis. {ECO:0000269|PubMed:30339758}.
nucleotide Binding
Features Chain (1); Region (1)
Keywords Reference proteome
Interact With
Induction INDUCTION: Expression is controlled by the asperlin biosynthesis cluster-specific transcription factor alnR. {ECO:0000269|PubMed:30339758}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 22,424
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda