| IED ID |
IndEnz0010000178 |
| Enzyme Type ID |
esterase000178 |
| Protein Name |
Asperlin biosynthesis cluster protein I
|
| Gene Name |
alnI ANIA_11200 |
| Organism |
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Pezizomycotina
leotiomyceta
Eurotiomycetes
Eurotiomycetidae
Eurotiales (green and blue molds)
Aspergillaceae
Aspergillus
Aspergillus subgen. Nidulantes
Emericella nidulans (Aspergillus nidulans)
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
|
| Enzyme Sequence |
MFSSTRRAEGPCATELTQVSSLLPPRGPYEFSLLPTLTRPLEDLSKCIEGARQTSATANGYSPTGLVPLADSILEICQAACTAYGLVDGAIAAGVGTGSSDNSPTATGIGAAGLTGDRPSSSGASTWRCVKTPMTLGSLTLQNEEESLLARQIVYAVLTSLSALLREVYVREKDVVSETDVVGEGGVGAGAALYGREGAGAVSQCLSRVLALLGKIVPE |
| Enzyme Length |
219 |
| Uniprot Accession Number |
C8VJQ9 |
| Absorption |
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| Active Site |
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| Activity Regulation |
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| Binding Site |
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| Calcium Binding |
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| catalytic Activity |
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| DNA Binding |
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| EC Number |
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| Enzyme Function |
FUNCTION: Part of the gene cluster that mediates the biosynthesis of asperlin, a polyketide showing anti-inflammatory, antitumor and antibiotic activities (PubMed:30339758). The first step of the asperlin biosynthesis is the production of the intermediate 2,4,6-octatrienoic acid by the highly redusing polyketide synthase alnA with cleavage of the PKS product by the esterase alnB (PubMed:30339758). 2,4,6-octatrienoic acid is further converted to asperlin via several steps involving the remaining enzymes from the cluster (PubMed:30339758). {ECO:0000269|PubMed:30339758}. |
| Temperature Dependency |
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| PH Dependency |
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| Pathway |
PATHWAY: Polyketide biosynthesis. {ECO:0000269|PubMed:30339758}. |
| nucleotide Binding |
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| Features |
Chain (1); Region (1) |
| Keywords |
Reference proteome |
| Interact With |
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| Induction |
INDUCTION: Expression is controlled by the asperlin biosynthesis cluster-specific transcription factor alnR. {ECO:0000269|PubMed:30339758}. |
| Subcellular Location |
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| Modified Residue |
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| Post Translational Modification |
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| Signal Peptide |
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| Structure 3D |
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| Cross Reference PDB |
- |
| Mapped Pubmed ID |
- |
| Motif |
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| Gene Encoded By |
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| Mass |
22,424 |
| Kinetics |
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| Metal Binding |
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| Rhea ID |
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| Cross Reference Brenda |
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