Detail Information for IndEnz0010000192
IED ID IndEnz0010000192
Enzyme Type ID esterase000192
Protein Name Pimeloyl-
acyl-carrier protein methyl ester esterase
EC 3.1.1.85
Biotin synthesis protein BioH
Carboxylesterase BioH
Gene Name bioH XF_1356
Organism Xylella fastidiosa (strain 9a5c)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Xanthomonadales Xanthomonadaceae Xylella Xylella fastidiosa Xylella fastidiosa (strain 9a5c)
Enzyme Sequence MYIEVTGYGPALVLIHGWAMHSGVFAPLVEQLRAHHTLYLVDLPGHGYNHTTLTPLALPHVVHAIAAATPPAVWLGWSLGGLFALHAAATLPQVRGLIMLAATPCFVRREDWPHAVEVSIFTQFAEDLKQNYTETINRFLALDTLGSTYAQSELRQLRQILNARHTPNTATLQAGLELLAHTDLRRAVIDLTPPSLWIAGQRDRLVPAASIHAATALAPSGQTELLTITGGGHAPFLSHANQMTAALQHFIATLP
Enzyme Length 255
Uniprot Accession Number Q9PDM3
Absorption
Active Site ACT_SITE 78; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 203; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 233; /evidence=ECO:0000255|HAMAP-Rule:MF_01260
Activity Regulation
Binding Site BINDING 18; /note=Substrate; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; BINDING 233; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_01260
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6-carboxyhexanoyl-[ACP] + H(+) + methanol; Xref=Rhea:RHEA:42700, Rhea:RHEA-COMP:9955, Rhea:RHEA-COMP:10186, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:78846, ChEBI:CHEBI:82735; EC=3.1.1.85; Evidence={ECO:0000255|HAMAP-Rule:MF_01260};
DNA Binding
EC Number 3.1.1.85
Enzyme Function FUNCTION: The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters. {ECO:0000255|HAMAP-Rule:MF_01260}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Cofactor biosynthesis; biotin biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01260}.
nucleotide Binding
Features Active site (3); Binding site (2); Chain (1); Region (2)
Keywords Biotin biosynthesis;Cytoplasm;Hydrolase;Reference proteome;Serine esterase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01260}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 27,627
Kinetics
Metal Binding
Rhea ID RHEA:42700
Cross Reference Brenda