Detail Information for IndEnz0010000197
IED ID IndEnz0010000197
Enzyme Type ID esterase000197
Protein Name Acetylcholinesterase
AChE
EC 3.1.1.7
Gene Name ache
Organism Danio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio)
Enzyme Sequence MKTSDILLLPTVLLTFLFHNCFAQAEPDLVVATRLGRVQGTRLPVPDRSHVIAFLGIPYAEPPIGKRRFKRAEPKKPWNNVFEAKEFSNACYQFVDTSYPGFPGIEMWNPNRVMSEDCLYLNVWVPPTPRPQNLTVMVWIYGGGFYSGSSSLDVYDGRYLAYTEKVVVVSMNYRVGAFGFLALNGSSDAPGNVGLYDQRLALQWVQENIHFFGGNPKQVTIFGESAGAASVGMHVLSPDSRPLFTRAILQSGVPNTPWATVTFDEARRRTTKLGKLVGCTWGNDTELIDCLRNKHPQELIDQEWQVLPWSSLFRFSFVPVVDGVFFPDTPDAMISSGNFKYTQILLGVNQDEGSYFLLYGAPGFSKDNESLISREDFLESVKMGVPHANDIGLEAVILQYTDWMDENNGQKNRDAMDDIVGDQNVICPLQHFAKSYAQYAALHAQSSAAAPGTLGWGNSGPTGYNSGNSHGAVYLYLFDHRASNLAWPEWMGVIHGYEIEFVFGLPLEKRLNYTAEEEKLSRRIMRYWANFARTGNPNVNTDGTMDSRRRWPQFSANEQKHVGLNTEPMKVHKGLRTQFCALWNRFLPRLLNITDNIDDVERQWKVEFHRWSSYMMHWKSQFDHYSKQERCTDL
Enzyme Length 634
Uniprot Accession Number Q9DDE3
Absorption
Active Site ACT_SITE 225; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 352; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 495; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=acetylcholine + H2O = acetate + choline + H(+); Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7; Evidence={ECO:0000269|PubMed:11016933};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17562; Evidence={ECO:0000305|PubMed:11016933};
DNA Binding
EC Number 3.1.1.7
Enzyme Function FUNCTION: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (4); Glycosylation (6); Signal peptide (1)
Keywords Cell junction;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Neurotransmitter degradation;Reference proteome;Secreted;Serine esterase;Signal;Synapse
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell junction, synapse. Secreted. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11753420; 15136152; 16425445; 16470869; 16806813; 17888594; 21844221; 21946396; 22459995; 25157430; 26528153; 29371671; 33499374; 9007258;
Motif
Gene Encoded By
Mass 71,998
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=230 uM for acetylcholine {ECO:0000269|PubMed:11016933};
Metal Binding
Rhea ID RHEA:17561; RHEA:17562
Cross Reference Brenda 3.1.1.7;