| IED ID | IndEnz0010000197 |
| Enzyme Type ID | esterase000197 |
| Protein Name |
Acetylcholinesterase AChE EC 3.1.1.7 |
| Gene Name | ache |
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio) |
| Enzyme Sequence | MKTSDILLLPTVLLTFLFHNCFAQAEPDLVVATRLGRVQGTRLPVPDRSHVIAFLGIPYAEPPIGKRRFKRAEPKKPWNNVFEAKEFSNACYQFVDTSYPGFPGIEMWNPNRVMSEDCLYLNVWVPPTPRPQNLTVMVWIYGGGFYSGSSSLDVYDGRYLAYTEKVVVVSMNYRVGAFGFLALNGSSDAPGNVGLYDQRLALQWVQENIHFFGGNPKQVTIFGESAGAASVGMHVLSPDSRPLFTRAILQSGVPNTPWATVTFDEARRRTTKLGKLVGCTWGNDTELIDCLRNKHPQELIDQEWQVLPWSSLFRFSFVPVVDGVFFPDTPDAMISSGNFKYTQILLGVNQDEGSYFLLYGAPGFSKDNESLISREDFLESVKMGVPHANDIGLEAVILQYTDWMDENNGQKNRDAMDDIVGDQNVICPLQHFAKSYAQYAALHAQSSAAAPGTLGWGNSGPTGYNSGNSHGAVYLYLFDHRASNLAWPEWMGVIHGYEIEFVFGLPLEKRLNYTAEEEKLSRRIMRYWANFARTGNPNVNTDGTMDSRRRWPQFSANEQKHVGLNTEPMKVHKGLRTQFCALWNRFLPRLLNITDNIDDVERQWKVEFHRWSSYMMHWKSQFDHYSKQERCTDL |
| Enzyme Length | 634 |
| Uniprot Accession Number | Q9DDE3 |
| Absorption | |
| Active Site | ACT_SITE 225; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 352; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 495; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetylcholine + H2O = acetate + choline + H(+); Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7; Evidence={ECO:0000269|PubMed:11016933};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17562; Evidence={ECO:0000305|PubMed:11016933}; |
| DNA Binding | |
| EC Number | 3.1.1.7 |
| Enzyme Function | FUNCTION: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (4); Glycosylation (6); Signal peptide (1) |
| Keywords | Cell junction;Cell membrane;Disulfide bond;Glycoprotein;Hydrolase;Membrane;Neurotransmitter degradation;Reference proteome;Secreted;Serine esterase;Signal;Synapse |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell junction, synapse. Secreted. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11753420; 15136152; 16425445; 16470869; 16806813; 17888594; 21844221; 21946396; 22459995; 25157430; 26528153; 29371671; 33499374; 9007258; |
| Motif | |
| Gene Encoded By | |
| Mass | 71,998 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=230 uM for acetylcholine {ECO:0000269|PubMed:11016933}; |
| Metal Binding | |
| Rhea ID | RHEA:17561; RHEA:17562 |
| Cross Reference Brenda | 3.1.1.7; |