IED Industry Enzyme Database

Detail Information for IndEnz0010000200
IED ID IndEnz0010000200
Enzyme Type ID esterase000200
Protein Name Cytosolic acyl coenzyme A thioester hydrolase
EC 3.1.2.2
Acyl-CoA thioesterase 7
Brain acyl-CoA hydrolase
BACH
CTE-IIa
CTE-II
Long chain acyl-CoA thioester hydrolase
Gene Name Acot7 Bach
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MKLLVGTLRLWEVGRQVAFSSLTPGQECSGLRKTFWAAMRAVRTRADHQKLGHCVTMGRIMRPDDANVAGNVHGGTILKMIEEAGAIISTRHCNSQNGERCVAALARVERTDFLSPMCIGEVAHVSAEITYTSKHSVEVQVHVMSENILTGTKKLTNKATLWYVPLSLKNVDKVLEVPPIVYLRQEQEEEGRKRYEAQKLERMETKWRNGDIVQPVLNPEPNTVSYSQSSLIHLVGPSDCTLHGFVHGGVTMKLMDEVAGIVAARHCKTNIVTASVDAINFHDKIRKGCVITISGRMTFTSNKSMEIEVLVDADPVVDNSQKRYRAASAFFTYVSLNQEGKPMPVPQLVPETEDEKKRFEEGKGRYLQMKAKRQGHTEPQP
Enzyme Length 381
Uniprot Accession Number Q91V12
Absorption
Active Site ACT_SITE 67; /evidence=ECO:0000269|PubMed:17563367; ACT_SITE 256; /evidence=ECO:0000269|PubMed:17563367
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate; Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=3.1.2.2; Evidence={ECO:0000269|PubMed:15288813};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646; Evidence={ECO:0000305|PubMed:15288813}; CATALYTIC ACTIVITY: Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+); Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375; Evidence={ECO:0000250|UniProtKB:Q64559};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30136; Evidence={ECO:0000250|UniProtKB:Q64559}; CATALYTIC ACTIVITY: Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate; Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385; Evidence={ECO:0000250|UniProtKB:Q64559};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120; Evidence={ECO:0000250|UniProtKB:Q64559}; CATALYTIC ACTIVITY: Reaction=decanoyl-CoA + H2O = CoA + decanoate + H(+); Xref=Rhea:RHEA:40059, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:27689, ChEBI:CHEBI:57287, ChEBI:CHEBI:61430; Evidence={ECO:0000250|UniProtKB:Q64559};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40060; Evidence={ECO:0000250|UniProtKB:Q64559}; CATALYTIC ACTIVITY: Reaction=H2O + octanoyl-CoA = CoA + H(+) + octanoate; Xref=Rhea:RHEA:30143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25646, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386; Evidence={ECO:0000250|UniProtKB:Q64559};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30144; Evidence={ECO:0000250|UniProtKB:Q64559}; CATALYTIC ACTIVITY: Reaction=H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate; Xref=Rhea:RHEA:30139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394; Evidence={ECO:0000250|UniProtKB:Q64559};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30140; Evidence={ECO:0000250|UniProtKB:Q64559}; CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+); Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387; Evidence={ECO:0000250|UniProtKB:Q64559};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40140; Evidence={ECO:0000250|UniProtKB:Q64559};
DNA Binding
EC Number 3.1.2.2
Enzyme Function FUNCTION: Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (Probable). Preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (Probable). May play an important physiological function in brain (Probable). {ECO:0000305|PubMed:15288813}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Lipid metabolism; fatty acid metabolism. {ECO:0000305|PubMed:15288813}.
nucleotide Binding
Features Active site (2); Alternative sequence (3); Beta strand (14); Chain (1); Compositional bias (1); Domain (2); Helix (6); Modified residue (3); Mutagenesis (2); Region (1); Sequence conflict (1); Turn (3)
Keywords 3D-structure;Acetylation;Alternative splicing;Cytoplasm;Direct protein sequencing;Fatty acid metabolism;Hydrolase;Lipid metabolism;Reference proteome;Repeat;Serine esterase
Interact With Itself
Induction INDUCTION: Up-Regulated in activated macrophages. {ECO:0000269|PubMed:17563367}.
Subcellular Location SUBCELLULAR LOCATION: [Isoform A]: Cytoplasm, cytosol {ECO:0000305|PubMed:15288813}.; SUBCELLULAR LOCATION: [Isoform D]: Cytoplasm, cytosol {ECO:0000305|PubMed:15288813}.
Modified Residue MOD_RES 169; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:O00154; MOD_RES 199; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:O00154; MOD_RES 284; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:O00154
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (4)
Cross Reference PDB 2Q2B; 2V1O; 4ZV3; 6VFY;
Mapped Pubmed ID 10725249; 11217851; 12466851; 12520002; 14610273; 14681479; 15226823; 16455042; 16602821; 16615898; 17514357; 17967808; 18324622; 18799693; 19455133; 21267068; 22034435; 23459938; 23649344; 25760036; 26861785; 26930384; 27133129; 28416579; 28705898; 31039008; 31676684;
Motif
Gene Encoded By
Mass 42,537
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=16 uM for palmitoyl-CoA (isoform A at 30 degrees Celsius) {ECO:0000269|PubMed:15288813}; KM=12 uM for palmitoyl-CoA (isoform D at 30 degrees Celsius) {ECO:0000269|PubMed:15288813};
Metal Binding
Rhea ID RHEA:16645; RHEA:16646; RHEA:30135; RHEA:30136; RHEA:40119; RHEA:40120; RHEA:40059; RHEA:40060; RHEA:30143; RHEA:30144; RHEA:30139; RHEA:30140; RHEA:40139; RHEA:40140
Cross Reference Brenda 3.1.2.2;3.1.2.20;