| IED ID | IndEnz0010000203 |
| Enzyme Type ID | esterase000203 |
| Protein Name |
Biotin biosynthesis bifunctional protein BioHC Includes: Carboxylesterase BioH EC 3.1.1.1 Biotin synthesis protein BioH ; Malonyl- acyl-carrier protein O-methyltransferase Malonyl-ACP O-methyltransferase EC 2.1.1.197 Biotin synthesis protein BioC |
| Gene Name | bioC CJA_0428 |
| Organism | Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Cellvibrionales Cellvibrionaceae Cellvibrio Cellvibrio japonicus (Pseudomonas fluorescens subsp. cellulosa) Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
| Enzyme Sequence | MTRPVLVLVHGWGCDSRTWQPVLDGLRELVPVQLVDLPGFGNTPALETFSLPAVLAAIESQLPERCVLLGWSLGAMLAVQLAARLPQQVLGVISLAANARFVASDDYPHAMAPDVNRQFNSRFAEQPQAALKMFTGLLAQGDVQERALLKQLRTQVPDAINHNWAQALQLLAELDNRAALVQLSQPLLHLLAEQDALVPIAAAESLRGLNSQHQIHVIAGSAHAVHWSQPQQLISAVQDFYETLATAVDKKRVAQSFGKAAATYDSVAGLQRAVGAQLLDYLPAQLDRTRLLDIGSGTGFFTAQLATRGAEVIALDIAQGMLDFARQQHPQAADWVCGDAENLPFAQSSVDFIFSSLVIQWCARVPQLMQELARVLKPGGRAYISTLGPGTLVELKRAWQQVDNYVHVNRFVGRTSLEQAVQQAGMQCLAFVESTRRLYFSRLRDLTHELKALGAHNINPGQAQGLTGRQRLQAFSLAYERERSPQGLPASYEVYYLVLCKP |
| Enzyme Length | 502 |
| Uniprot Accession Number | B3PI89 |
| Absorption | |
| Active Site | ACT_SITE 72; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 195; /evidence=ECO:0000250; ACT_SITE 223; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | BINDING 12; /note=Substrate; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000250; BINDING 223; /note=Substrate; /evidence=ECO:0000250 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; CATALYTIC ACTIVITY: Reaction=malonyl-[ACP] + S-adenosyl-L-methionine = malonyl-[ACP] methyl ester + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:17105, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9954, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:78449, ChEBI:CHEBI:78845; EC=2.1.1.197; |
| DNA Binding | |
| EC Number | 3.1.1.1; 2.1.1.197 |
| Enzyme Function | FUNCTION: Converts the free carboxyl group of a malonyl-thioester to its methyl ester by transfer of a methyl group from S-adenosyl-L-methionine (SAM). It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway (By similarity). {ECO:0000250}.; FUNCTION: The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Cofactor biosynthesis; biotin biosynthesis. |
| nucleotide Binding | |
| Features | Active site (3); Binding site (2); Chain (1); Region (3) |
| Keywords | Biotin biosynthesis;Hydrolase;Methyltransferase;Multifunctional enzyme;Reference proteome;S-adenosyl-L-methionine;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,021 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21164; RHEA:17105 |
| Cross Reference Brenda |