IED ID | IndEnz0010000204 |
Enzyme Type ID | esterase000204 |
Protein Name |
Biotin biosynthesis bifunctional protein BioHC Includes: Carboxylesterase BioH EC 3.1.1.1 Biotin synthesis protein BioH ; Malonyl- acyl-carrier protein O-methyltransferase Malonyl-ACP O-methyltransferase EC 2.1.1.197 Biotin synthesis protein BioC |
Gene Name | bioC Sde_3137 |
Organism | Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) |
Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Cellvibrionales Cellvibrionaceae Saccharophagus Saccharophagus degradans Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) |
Enzyme Sequence | MSNIEPSNVKPSNIELGTIPLAEAPATKLEAAYIAKKIAPNNSNPNAPVWVFVHGWGASANTWAPLVDELKSQCEIWLLDLPSFGGNTQAVNSPSAVAADIAKILPANTVLVGWSLGGMLLPLIAQQIEQHWPTKTISHCIGLAANAKFAQGDNYTAAMPAETFQTFCANFNVDPTTTWSRFGLLQAQGDSNRKLVSRQIKALHNAPMPEQFTAWQQALTWLGAIDNRVLLCEITTPFLHLFGEGDALVPADAAVAMAGINPLHQTLVVASAGHVLHFSQPAKIAQIMLARVHAKRNKARVAKSFSNAATEYDSVAYLQQKLANTLCEWVPEQAEKIADLGCGTGYCGLQLQRPERDIYSLDLAQGMLHTARSKALAKQQLFSGVCADIECLPFISNGFDALVSGMSMQWCEDLPAVFSEAHRVLKPNGEMIFSTLGPQTLFELREAWAEADIKLGRQGCVHVNTFIELDRVEIAAKQAGFVIEQTSREIHVLTYDSVMPLMRELKTIGAHNVNPGQEQGLTGKARLKAMAQAYEQFRTAQGVLPLTYEVGFYQLLKV |
Enzyme Length | 558 |
Uniprot Accession Number | Q21FY5 |
Absorption | |
Active Site | ACT_SITE 115; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 246; /evidence=ECO:0000250; ACT_SITE 274; /evidence=ECO:0000250 |
Activity Regulation | |
Binding Site | BINDING 56; /note=Substrate; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000250; BINDING 274; /note=Substrate; /evidence=ECO:0000250 |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; CATALYTIC ACTIVITY: Reaction=malonyl-[ACP] + S-adenosyl-L-methionine = malonyl-[ACP] methyl ester + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:17105, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9954, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:78449, ChEBI:CHEBI:78845; EC=2.1.1.197; |
DNA Binding | |
EC Number | 3.1.1.1; 2.1.1.197 |
Enzyme Function | FUNCTION: Converts the free carboxyl group of a malonyl-thioester to its methyl ester by transfer of a methyl group from S-adenosyl-L-methionine (SAM). It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway (By similarity). {ECO:0000250}.; FUNCTION: The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters (By similarity). {ECO:0000250}. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Cofactor biosynthesis; biotin biosynthesis. |
nucleotide Binding | |
Features | Active site (3); Binding site (2); Chain (1); Region (4) |
Keywords | Biotin biosynthesis;Hydrolase;Methyltransferase;Multifunctional enzyme;Reference proteome;S-adenosyl-L-methionine;Transferase |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 60,825 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:21164; RHEA:17105 |
Cross Reference Brenda |