| IED ID | IndEnz0010000231 |
| Enzyme Type ID | esterase000231 |
| Protein Name |
Pimeloyl- acyl-carrier protein methyl ester esterase EC 3.1.1.85 Biotin synthesis protein BioH Carboxylesterase BioH |
| Gene Name | bioH HDEF_0547 |
| Organism | Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Enterobacteriaceae incertae sedis ant tsetse mealybug aphid etc. endosymbionts aphid secondary symbionts Candidatus Hamiltonella Candidatus Hamiltonella defensa Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) |
| Enzyme Sequence | MKNLYRYSCGEGKLDFVMLHGWGMNSNAWRYIINRFGSHFRLHLFDLPGYGFSQSEESFTLPEISETVLKKAPPQAIWLGWSMGGCIASEIALRYPERVLALITVCSSPCFVSQHQWPRISWQVLSCFEQKLEDHLQNTLEQFLLLQTLGTLNSQRDTTSLKSFLFSRPLPKKEVLKAGLKILRYTDMRDSLNAISVPFLRIYGKLDALVPCKIAELLDEAWPHTESVIMHQSAHVPFISHTDDFLEVILYFYQKYFSH |
| Enzyme Length | 259 |
| Uniprot Accession Number | C4K407 |
| Absorption | |
| Active Site | ACT_SITE 82; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 207; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; ACT_SITE 235; /evidence=ECO:0000255|HAMAP-Rule:MF_01260 |
| Activity Regulation | |
| Binding Site | BINDING 22; /note=Substrate; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_01260; BINDING 235; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_01260 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=6-carboxyhexanoyl-[ACP] methyl ester + H2O = 6-carboxyhexanoyl-[ACP] + H(+) + methanol; Xref=Rhea:RHEA:42700, Rhea:RHEA-COMP:9955, Rhea:RHEA-COMP:10186, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:78846, ChEBI:CHEBI:82735; EC=3.1.1.85; Evidence={ECO:0000255|HAMAP-Rule:MF_01260}; |
| DNA Binding | |
| EC Number | 3.1.1.85 |
| Enzyme Function | FUNCTION: The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters. {ECO:0000255|HAMAP-Rule:MF_01260}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Cofactor biosynthesis; biotin biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01260}. |
| nucleotide Binding | |
| Features | Active site (3); Binding site (2); Chain (1); Domain (1); Region (2) |
| Keywords | Biotin biosynthesis;Cytoplasm;Hydrolase;Reference proteome;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01260}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 29,957 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:42700 |
| Cross Reference Brenda |