IED ID | IndEnz0010000315 |
Enzyme Type ID | esterase000315 |
Protein Name |
Probable carboxylesterase 12 AtCXE12 EC 3.1.1.1 |
Gene Name | CXE12 At3g48690 T8P19.200 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MDSEIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLKPECDNAIEVMHKFSGFIKGGN |
Enzyme Length | 324 |
Uniprot Accession Number | Q9SMN0 |
Absorption | |
Active Site | ACT_SITE 162; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038; ACT_SITE 265; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038; ACT_SITE 297; /evidence=ECO:0000255|PROSITE-ProRule:PRU10038 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; |
DNA Binding | |
EC Number | 3.1.1.1 |
Enzyme Function | FUNCTION: Carboxylesterase acting on esters with varying acyl chain length. {ECO:0000250}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Chain (1); Modified residue (1); Motif (1); Sequence conflict (2) |
Keywords | Acetylation;Hydrolase;Reference proteome;Serine esterase |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | MOD_RES 1; /note=N-acetylmethionine; /evidence=ECO:0007744|PubMed:22223895 |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 11351099; 12368499; 14982972; 17519238; 18493073; 18775970; 19136719; 21763150; 21764588; 21798944; 28627464; |
Motif | MOTIF 78..80; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000250|UniProtKB:Q5NUF3 |
Gene Encoded By | |
Mass | 35,567 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:21164 |
Cross Reference Brenda |