| IED ID | IndEnz0010000339 |
| Enzyme Type ID | esterase000339 |
| Protein Name |
Acyl-coenzyme A thioesterase 3 Acyl-CoA thioesterase 3 EC 3.1.2.2 PTE-Ia Peroxisomal acyl-coenzyme A thioester hydrolase 2a Peroxisomal long-chain acyl-CoA thioesterase 2 |
| Gene Name | Acot3 Pte1a Pte2 Pte2a |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MHAFTTQNPNRMAPTVILEPAGGCLCDQPVHIAVRGLAPEQPVTLRSVLRDEKGALFRAHARYRADSHGELDLARTPALGGSFSGLEPMGLLWAMEPDRPFWRLIKRDVQTPFVVELEVLDGHEPDGGQRLARAVHERHFMAPGVRRVPVREGRVRATLFLPPGTGPFPGIIDLFGIGSGLLEYRASLLAGKGFAVMALAYNNYEDLPKDMDIIHLEYFEEAVTYLLSHPQVTGSGVGVLGISKGGELGFAMASFLKNITAAVIINGSISNIGGNLQYKDETVPSVGINTKRVKRTKDGLKDIVDLLNNPLEGPDQKSLIPVERSDTAFLFLVGQDDHNWKSEFYAREASKRLQAHGKEKPQIICYPETGHHIEPPYFPLCKASLNSLVGGPVIWGGEPRAHAMAQVDAWQQLQTFFHNHLDGKKKTIPAKL |
| Enzyme Length | 432 |
| Uniprot Accession Number | Q9QYR7 |
| Absorption | |
| Active Site | ACT_SITE 243; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 337; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 371; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate; Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=3.1.2.2; Evidence={ECO:0000269|PubMed:15007068};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=decanoyl-CoA + H2O = CoA + decanoate + H(+); Xref=Rhea:RHEA:40059, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:27689, ChEBI:CHEBI:57287, ChEBI:CHEBI:61430; Evidence={ECO:0000269|PubMed:15007068, ECO:0000269|PubMed:16940157};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40060; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+); Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375; Evidence={ECO:0000269|PubMed:15007068, ECO:0000269|PubMed:16940157};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30136; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate; Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385; Evidence={ECO:0000269|PubMed:15007068, ECO:0000269|PubMed:16940157};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate; Xref=Rhea:RHEA:30139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394; Evidence={ECO:0000269|PubMed:15007068, ECO:0000269|PubMed:16940157};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30140; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=eicosanoyl-CoA + H2O = CoA + eicosanoate + H(+); Xref=Rhea:RHEA:40147, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32360, ChEBI:CHEBI:57287, ChEBI:CHEBI:57380; Evidence={ECO:0000269|PubMed:15007068, ECO:0000269|PubMed:16940157};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40148; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+); Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387; Evidence={ECO:0000269|PubMed:15007068, ECO:0000269|PubMed:16940157};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40140; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=(9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate + CoA + H(+); Xref=Rhea:RHEA:40143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30245, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383; Evidence={ECO:0000269|PubMed:15007068, ECO:0000269|PubMed:16940157};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40144; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H(+); Xref=Rhea:RHEA:40151, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32395, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368; Evidence={ECO:0000269|PubMed:15007068, ECO:0000269|PubMed:16940157};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40152; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=H2O + tetracosanoyl-CoA = CoA + H(+) + tetracosanoate; Xref=Rhea:RHEA:40787, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:31014, ChEBI:CHEBI:57287, ChEBI:CHEBI:65052; Evidence={ECO:0000269|PubMed:15007068};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40788; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=H2O + hexacosanoyl-CoA = CoA + H(+) + hexacosanoate; Xref=Rhea:RHEA:40791, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:31013, ChEBI:CHEBI:57287, ChEBI:CHEBI:64868; Evidence={ECO:0000269|PubMed:15007068};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40792; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=docosanoyl-CoA + H2O = CoA + docosanoate + H(+); Xref=Rhea:RHEA:40783, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:23858, ChEBI:CHEBI:57287, ChEBI:CHEBI:65059; Evidence={ECO:0000269|PubMed:15007068};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40784; Evidence={ECO:0000305|PubMed:15007068}; CATALYTIC ACTIVITY: Reaction=(9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H(+); Xref=Rhea:RHEA:40131, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:57287, ChEBI:CHEBI:61540; Evidence={ECO:0000269|PubMed:15007068};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40132; Evidence={ECO:0000305|PubMed:15007068}; |
| DNA Binding | |
| EC Number | 3.1.2.2 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:15007068, PubMed:16940157). Mainly active on long-chain acyl-CoAs (PubMed:15007068, PubMed:16940157). May have a function in termination of beta-oxidation of fatty acids (PubMed:16940157). {ECO:0000269|PubMed:15007068, ECO:0000269|PubMed:16940157}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Lipid metabolism; fatty acid metabolism. {ECO:0000305|PubMed:15007068, ECO:0000305|PubMed:16940157}. |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Chain (1); Motif (1) |
| Keywords | Alternative splicing;Fatty acid metabolism;Hydrolase;Lipid metabolism;Peroxisome;Reference proteome;Serine esterase |
| Interact With | |
| Induction | INDUCTION: In the liver and kidney, by peroxisome proliferator (such as Clofibrate) treatment, via the peroxisome proliferator-activated receptors (PPARs) or fasting for 24 hours. |
| Subcellular Location | SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:15007068}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14681479; |
| Motif | MOTIF 430..432; /note=Microbody targeting signal; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 47,490 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=10.9 uM for hexanoyl-coA {ECO:0000269|PubMed:15007068}; KM=46.2 uM for octanoyl-coA {ECO:0000269|PubMed:15007068}; KM=7.7 uM for decanoyl-coA {ECO:0000269|PubMed:15007068}; KM=7.6 uM for dodecanoyl-coA {ECO:0000269|PubMed:15007068}; KM=3.8 uM for tetradecanoyl-coA {ECO:0000269|PubMed:15007068}; KM=4.0 uM for hexadecanoyl-coA {ECO:0000269|PubMed:15007068}; KM=2.8 uM for (9Z)-hexadecenoyl-coA {ECO:0000269|PubMed:15007068}; KM=4.2 uM for octadecanoyl-coA {ECO:0000269|PubMed:15007068}; KM=4.6 uM for (9Z)-octadecenoyl-coA {ECO:0000269|PubMed:15007068}; KM=2.6 uM for (9Z,12Z)-octadecadienoyl-coA {ECO:0000269|PubMed:15007068}; KM=2.2 uM for eicosanoyl-coA {ECO:0000269|PubMed:15007068}; KM=7.2 uM for (5Z,8Z,11Z,14Z)-eicosatetraenoyl-coA {ECO:0000269|PubMed:15007068}; KM=2.3 uM for docosanoyl-coA {ECO:0000269|PubMed:15007068}; KM=6.3 uM for tetracosanoyl-coA {ECO:0000269|PubMed:15007068}; KM=4.5 uM for hexacosanoyl-coA {ECO:0000269|PubMed:15007068}; Vmax=0.02 umol/min/mg enzyme with hexanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.23 umol/min/mg enzyme with octanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.87 umol/min/mg enzyme with decanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=1.40 umol/min/mg enzyme with dodecanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=1.60 umol/min/mg enzyme with tetradecanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=1.85 umol/min/mg enzyme with hexadecanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.93 umol/min/mg enzyme with (9Z)-hexadecenoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=1.43 umol/min/mg enzyme with octadecanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.85 umol/min/mg enzyme with (9Z)-octadecenoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.63 umol/min/mg enzyme with (9Z,12Z)-octadecadienoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.71 umol/min/mg enzyme with eicosanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.70 umol/min/mg enzyme with (5Z,8Z,11Z,14Z)-eicosatetraenoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.37 umol/min/mg enzyme with docosanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.20 umol/min/mg enzyme with tetracosanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; Vmax=0.22 umol/min/mg enzyme with hexacosanoyl-coA as substrate {ECO:0000269|PubMed:15007068}; |
| Metal Binding | |
| Rhea ID | RHEA:16645; RHEA:16646; RHEA:40059; RHEA:40060; RHEA:30135; RHEA:30136; RHEA:40119; RHEA:40120; RHEA:30139; RHEA:30140; RHEA:40147; RHEA:40148; RHEA:40139; RHEA:40140; RHEA:40143; RHEA:40144; RHEA:40151; RHEA:40152; RHEA:40787; RHEA:40788; RHEA:40791; RHEA:40792; RHEA:40783; RHEA:40784; RHEA:40131; RHEA:40132 |
| Cross Reference Brenda |