IED Industry Enzyme Database

Detail Information for IndEnz0010000350
IED ID IndEnz0010000350
Enzyme Type ID esterase000350
Protein Name Acyl-coenzyme A thioesterase 8
Acyl-CoA thioesterase 8
EC 3.1.2.1
EC 3.1.2.11
EC 3.1.2.2
EC 3.1.2.3
EC 3.1.2.5
Choloyl-coenzyme A thioesterase
EC 3.1.2.27
Peroxisomal acyl-CoA thioesterase 2
PTE-2
Peroxisomal acyl-coenzyme A thioester hydrolase 1
PTE-1
Peroxisomal long-chain acyl-CoA thioesterase 1
Gene Name Acot8 Pte1
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MSAPEGLGDAHGDADRGDLSGDLRSVLVTSVLNLEPLDEDLYRGRHYWVPTSQRLFGGQIMGQALVAAAKSVSEDVHVHSLHCYFVRAGDPKVPVLYHVERIRTGASFSVRAVKAVQHGKAIFICQASFQQMQPSPLQHQFSMPSVPPPEDLLDHEALIDQYLRDPNLHKKYRVGLNRVAAQEVPIEIKVVNPPTLTQLQALEPKQMFWVRARGYIGEGDIKMHCCVAAYISDYAFLGTALLPHQSKYKVNFMASLDHSMWFHAPFRADHWMLYECESPWAGGSRGLVHGRLWRRDGVLAVTCAQEGVIRLKPQVSESKL
Enzyme Length 320
Uniprot Accession Number P58137
Absorption
Active Site ACT_SITE 233; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 255; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 305; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation ACTIVITY REGULATION: Inhibited by CoASH (IC(50)=10-15 uM). Also inhibited by cysteine-reactive agents. {ECO:0000269|PubMed:11673457}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=choloyl-CoA + H2O = cholate + CoA + H(+); Xref=Rhea:RHEA:14541, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29747, ChEBI:CHEBI:57287, ChEBI:CHEBI:57373; EC=3.1.2.27; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14542; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=chenodeoxycholoyl-CoA + H2O = chenodeoxycholate + CoA + H(+); Xref=Rhea:RHEA:31511, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:36234, ChEBI:CHEBI:57287, ChEBI:CHEBI:62989; EC=3.1.2.27; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31512; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20290; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=H2O + malonyl-CoA = CoA + H(+) + malonate; Xref=Rhea:RHEA:40219, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15792, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384; Evidence={ECO:0000269|PubMed:11673457, ECO:0000269|PubMed:16141203};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40220; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=acetoacetyl-CoA + H2O = acetoacetate + CoA + H(+); Xref=Rhea:RHEA:15673, ChEBI:CHEBI:13705, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57286, ChEBI:CHEBI:57287; EC=3.1.2.11; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15674; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=H2O + propanoyl-CoA = CoA + H(+) + propanoate; Xref=Rhea:RHEA:40103, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17272, ChEBI:CHEBI:57287, ChEBI:CHEBI:57392; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40104; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=butanoyl-CoA + H2O = butanoate + CoA + H(+); Xref=Rhea:RHEA:40111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:57287, ChEBI:CHEBI:57371; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40112; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=H2O + succinyl-CoA = CoA + H(+) + succinate; Xref=Rhea:RHEA:11516, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30031, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292; EC=3.1.2.3; Evidence={ECO:0000269|PubMed:16141203};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11517; Evidence={ECO:0000305|PubMed:16141203}; CATALYTIC ACTIVITY: Reaction=glutaryl-CoA + H2O = CoA + glutarate + H(+); Xref=Rhea:RHEA:40575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30921, ChEBI:CHEBI:57287, ChEBI:CHEBI:57378; Evidence={ECO:0000269|PubMed:16141203};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40576; Evidence={ECO:0000305|PubMed:16141203}; CATALYTIC ACTIVITY: Reaction=H2O + hexanoyl-CoA = CoA + H(+) + hexanoate; Xref=Rhea:RHEA:40115, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17120, ChEBI:CHEBI:57287, ChEBI:CHEBI:62620; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40116; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=H2O + hexanedioyl-CoA = CoA + H(+) + hexanedioate; Xref=Rhea:RHEA:40583, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17128, ChEBI:CHEBI:57287, ChEBI:CHEBI:76327; Evidence={ECO:0000269|PubMed:16141203};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40584; Evidence={ECO:0000305|PubMed:16141203}; CATALYTIC ACTIVITY: Reaction=H2O + octanoyl-CoA = CoA + H(+) + octanoate; Xref=Rhea:RHEA:30143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25646, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30144; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=H2O + octanedioyl-CoA = CoA + H(+) + octanedioate; Xref=Rhea:RHEA:40587, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:76282, ChEBI:CHEBI:76317; Evidence={ECO:0000269|PubMed:16141203};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40588; Evidence={ECO:0000305|PubMed:16141203}; CATALYTIC ACTIVITY: Reaction=decanoyl-CoA + H2O = CoA + decanoate + H(+); Xref=Rhea:RHEA:40059, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:27689, ChEBI:CHEBI:57287, ChEBI:CHEBI:61430; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40060; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=decanedioyl-CoA + H2O = CoA + decanedioate + H(+); Xref=Rhea:RHEA:40591, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:76283, ChEBI:CHEBI:76316; Evidence={ECO:0000269|PubMed:16141203};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40592; Evidence={ECO:0000305|PubMed:16141203}; CATALYTIC ACTIVITY: Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+); Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30136; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=dodecanedioyl-CoA + H2O = CoA + dodecanedioate + H(+); Xref=Rhea:RHEA:40595, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:76273, ChEBI:CHEBI:76315; Evidence={ECO:0000269|PubMed:16141203};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40596; Evidence={ECO:0000305|PubMed:16141203}; CATALYTIC ACTIVITY: Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate; Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=(9Z)-tetradecenoyl-CoA + H2O = (9Z)-tetradecenoate + CoA + H(+); Xref=Rhea:RHEA:40135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32370, ChEBI:CHEBI:57287, ChEBI:CHEBI:65060; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40136; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate; Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=3.1.2.2; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=(9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H(+); Xref=Rhea:RHEA:40131, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:57287, ChEBI:CHEBI:61540; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40132; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate; Xref=Rhea:RHEA:30139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30140; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+); Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40140; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=(9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate + CoA + H(+); Xref=Rhea:RHEA:40143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30245, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40144; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=eicosanoyl-CoA + H2O = CoA + eicosanoate + H(+); Xref=Rhea:RHEA:40147, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32360, ChEBI:CHEBI:57287, ChEBI:CHEBI:57380; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40148; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H(+); Xref=Rhea:RHEA:40151, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32395, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40152; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=4,8-dimethylnonanoyl-CoA + H2O = 4,8-dimethylnonanoate + CoA + H(+); Xref=Rhea:RHEA:40223, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:77061, ChEBI:CHEBI:77063; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40224; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=2,6-dimethylheptanoyl-CoA + H2O = 2,6-dimethylheptanoate + CoA + H(+); Xref=Rhea:RHEA:59952, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:84847, ChEBI:CHEBI:143533; Evidence={ECO:0000250|UniProtKB:Q8VHK0};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59953; Evidence={ECO:0000250|UniProtKB:Q8VHK0}; CATALYTIC ACTIVITY: Reaction=(3S)-hydroxy-3-methylglutaryl-CoA + H2O = 3-hydroxy-3-methylglutarate + CoA + H(+); Xref=Rhea:RHEA:16305, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17325, ChEBI:CHEBI:43074, ChEBI:CHEBI:57287; EC=3.1.2.5; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16306; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA + H(+); Xref=Rhea:RHEA:59936, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:63001, ChEBI:CHEBI:85674; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59937; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=2-methyloctadecanoyl-CoA + H2O = 2-methyloctadecanoate + CoA + H(+); Xref=Rhea:RHEA:59940, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:143530, ChEBI:CHEBI:143531; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59941; Evidence={ECO:0000305|PubMed:11673457}; CATALYTIC ACTIVITY: Reaction=H2O + prostaglandin F2alpha-CoA = CoA + H(+) + prostaglandin F2alpha; Xref=Rhea:RHEA:59948, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57404, ChEBI:CHEBI:143532; Evidence={ECO:0000269|PubMed:11673457};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59949; Evidence={ECO:0000305|PubMed:11673457};
DNA Binding
EC Number 3.1.2.1; 3.1.2.11; 3.1.2.2; 3.1.2.3; 3.1.2.5; 3.1.2.27
Enzyme Function FUNCTION: Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:11673457). Displays no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (PubMed:11673457). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains (PubMed:11673457). Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (PubMed:11673457). Is also able to hydrolyze CoA esters of dicarboxylic acids (PubMed:16141203). It is involved in the metabolic regulation of peroxisome proliferation (By similarity). {ECO:0000250|UniProtKB:O14734, ECO:0000269|PubMed:11673457, ECO:0000269|PubMed:16141203}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Lipid metabolism; fatty acid metabolism. {ECO:0000305|PubMed:11673457}.
nucleotide Binding
Features Active site (3); Chain (1); Motif (1); Sequence conflict (1)
Keywords Fatty acid metabolism;Hydrolase;Lipid metabolism;Peroxisome;Peroxisome biogenesis;Reference proteome;Serine esterase
Interact With
Induction INDUCTION: Induced in the liver, by peroxisome proliferator or fasting via the peroxisome proliferator-activated receptors (PPARs). Diurnal regulation of its expression. {ECO:0000269|PubMed:11673457}.
Subcellular Location SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000269|PubMed:11673457}. Note=Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded. {ECO:0000250|UniProtKB:O14734}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 14561759; 14610273; 14651853; 14681479; 16103133; 16141072; 21267068; 21677750;
Motif MOTIF 318..320; /note=Microbody targeting signal; /evidence=ECO:0000255
Gene Encoded By
Mass 35,827
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=29.4 uM for acetyl-CoA {ECO:0000269|PubMed:11673457}; KM=8.0 uM for propionyl-CoA {ECO:0000269|PubMed:11673457}; KM=22.6 uM for butyryl-CoA {ECO:0000269|PubMed:11673457}; KM=23.4 uM for hexanoyl-CoA {ECO:0000269|PubMed:11673457}; KM=6.9 uM for octanoyl-CoA {ECO:0000269|PubMed:11673457}; KM=2.9 uM for decanoyl-CoA {ECO:0000269|PubMed:11673457}; KM=2.8 uM for lauroyl-CoA {ECO:0000269|PubMed:11673457}; KM=2.5 uM for myristoyl-CoA {ECO:0000269|PubMed:11673457}; KM=3.5 uM for myristoleoyl-CoA {ECO:0000269|PubMed:11673457}; KM=1.7 uM for palmitoyl-CoA {ECO:0000269|PubMed:11673457}; KM=1.4 uM for palmitoleoyl-CoA {ECO:0000269|PubMed:11673457}; KM=2.7 uM for stearoyl-CoA {ECO:0000269|PubMed:11673457}; KM=1.6 uM for oleoyl-CoA {ECO:0000269|PubMed:11673457}; KM=2.3 uM for linoleoyl-CoA {ECO:0000269|PubMed:11673457}; KM=4.2 uM for arachidoyl-CoA {ECO:0000269|PubMed:11673457}; KM=6.7 uM for arachidonoyl-CoA {ECO:0000269|PubMed:11673457}; KM=6.3 uM for 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA {ECO:0000269|PubMed:11673457}; KM=14.6 uM for choloyl-CoA {ECO:0000269|PubMed:11673457}; KM=8.8 uM for chenodeoxycholoyl-CoA {ECO:0000269|PubMed:11673457}; KM=5.5 uM for 4,8-dimethylnonanoyl-CoA {ECO:0000269|PubMed:11673457}; KM=0.92 uM for prostaglandin F2alpha-CoA {ECO:0000269|PubMed:11673457}; KM=1.9 uM for 2-methyloctadecanoyl-CoA {ECO:0000269|PubMed:11673457}; KM=22.9 uM for (3S)-hydroxy-3-methylglutaryl-CoA {ECO:0000269|PubMed:11673457}; KM=12.9 uM for malonyl-CoA {ECO:0000269|PubMed:11673457}; KM=22.9 uM for acetoacetyl-CoA {ECO:0000269|PubMed:11673457}; KM=34.7 uM for succinyl-CoA {ECO:0000269|PubMed:16141203}; KM=15.9 uM for glutaryl-CoA {ECO:0000269|PubMed:16141203}; KM=21.8 uM for hexanedioyl-CoA {ECO:0000269|PubMed:16141203}; KM=19.8 uM for octanoyl-CoA {ECO:0000269|PubMed:16141203}; KM=10.5 uM for decanedioyl-CoA {ECO:0000269|PubMed:16141203}; KM=14.1 uM for dodecanedioyl-CoA {ECO:0000269|PubMed:16141203}; Vmax=4.6 umol/min/mg enzyme with acetyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=3.45 umol/min/mg enzyme with propionyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=2.6 umol/min/mg enzyme with butyryl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=5.6 umol/min/mg enzyme with hexanoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=3.9 umol/min/mg enzyme with octanoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=3.8 umol/min/mg enzyme with decanoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=3.0 umol/min/mg enzyme with lauroyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=3.6 umol/min/mg enzyme with myristoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=3.2 umol/min/mg enzyme with myristoleoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=1.7 umol/min/mg enzyme with palmitoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=2.7 umol/min/mg enzyme with palmitoleoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=3.2 umol/min/mg enzyme with stearoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=3.1 umol/min/mg enzyme with oleoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=2.3 umol/min/mg enzyme with linoleoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=2.2 umol/min/mg enzyme with arachidoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=1.6 umol/min/mg enzyme with arachidonoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=8.1 umol/min/mg enzyme with 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=17.9 umol/min/mg enzyme with choloyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=17.1 umol/min/mg enzyme with chenodeoxycholoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=10.69 umol/min/mg enzyme with 4,8-dimethylnonanoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=2.25 umol/min/mg enzyme with prostaglandin F2alpha-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=1.65 umol/min/mg enzyme with 2-methyloctadecanoyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=2.85 umol/min/mg enzyme with (3S)-hydroxy-3-methylglutaryl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=1.19 umol/min/mg enzyme with malonyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=6.0 umol/min/mg enzyme with acetoacetyl-CoA as substrate {ECO:0000269|PubMed:11673457}; Vmax=0.82 umol/min/mg enzyme with succinyl-CoA as substrate {ECO:0000269|PubMed:16141203}; Vmax=2.35 umol/min/mg enzyme with glutaryl-CoA as substrate {ECO:0000269|PubMed:16141203}; Vmax=3.4 umol/min/mg enzyme with hexanedioyl-CoA as substrate {ECO:0000269|PubMed:16141203}; Vmax=4.13 umol/min/mg enzyme with octanoyl-CoA as substrate {ECO:0000269|PubMed:16141203}; Vmax=3.82 umol/min/mg enzyme with decanedioyl-CoA as substrate {ECO:0000269|PubMed:16141203}; Vmax=5.03 umol/min/mg enzyme with dodecanedioyl-CoA as substrate {ECO:0000269|PubMed:16141203}; Note=In summary, KM for medium- to long-chain acyl CoAs is in the order 1.4-6.7 uM, and with short-chain acyl CoAs range from 8 to 30 uM. KM for bile acid-CoA esters is in the range 6-15 uM. {ECO:0000269|PubMed:11673457};
Metal Binding
Rhea ID RHEA:14541; RHEA:14542; RHEA:31511; RHEA:31512; RHEA:20289; RHEA:20290; RHEA:40219; RHEA:40220; RHEA:15673; RHEA:15674; RHEA:40103; RHEA:40104; RHEA:40111; RHEA:40112; RHEA:11516; RHEA:11517; RHEA:40575; RHEA:40576; RHEA:40115; RHEA:40116; RHEA:40583; RHEA:40584; RHEA:30143; RHEA:30144; RHEA:40587; RHEA:40588; RHEA:40059; RHEA:40060; RHEA:40591; RHEA:40592; RHEA:30135; RHEA:30136; RHEA:40595; RHEA:40596; RHEA:40119; RHEA:40120; RHEA:40135; RHEA:40136; RHEA:16645; RHEA:16646; RHEA:40131; RHEA:40132; RHEA:30139; RHEA:30140; RHEA:40139; RHEA:40140; RHEA:40143; RHEA:40144; RHEA:40147; RHEA:40148; RHEA:40151; RHEA:40152; RHEA:40223; RHEA:40224; RHEA:59952; RHEA:59953; RHEA:16305; RHEA:16306; RHEA:59936; RHEA:59937; RHEA:59940; RHEA:59941; RHEA:59948; RHEA:59949
Cross Reference Brenda 3.1.2.2;3.1.2.20;3.1.2.27;