| IED ID | IndEnz0010000352 |
| Enzyme Type ID | esterase000352 |
| Protein Name |
Jasmonate monooxygenase ABM EC 1.14.13.228 Antibiotic biosynthesis monooxygenase ABM |
| Gene Name | ABM MGG_04777 |
| Organism | Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Sordariomycetidae Magnaporthales Pyriculariaceae Pyricularia Magnaporthe oryzae (Rice blast fungus) (Pyricularia oryzae) Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
| Enzyme Sequence | MFAVIFETRPQPSQFDTYLTIAKSLRPELANIDGFIENIRYKSLSRPGWILSLSFWRDEKSLVRWRTTATHHMAQEKGRDGVLEDYHLRVGQVTASVRSNEVKRVETVDAREQDDVTAVGAAKTVQLVRFQMDRGAGIDAAAGKLGLNPEEPRGLLAWDIMEAVLSPGDMILLASWETGSSSVSIPGADSDEVQVLRDYGKYDRREAPQFYPPAS |
| Enzyme Length | 215 |
| Uniprot Accession Number | P0CU79 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H(+) + jasmonate + NADPH + O2 = (1R,2R)-12-hydroxyjasmonate + H2O + NADP(+); Xref=Rhea:RHEA:49984, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58431, ChEBI:CHEBI:132022; EC=1.14.13.228; Evidence={ECO:0000269|PubMed:26258762};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:49985; Evidence={ECO:0000269|PubMed:26258762}; |
| DNA Binding | |
| EC Number | 1.14.13.228 |
| Enzyme Function | FUNCTION: Monooxygenase that converts the endogenous (and likely the host) jasmonate (JA) to its hydroxylated derivative 12-hydroxyjasmonate (12OH-JA), also known as tuberonic acid, a compound that attenuates or disables jasmonate-based host innate immunity and which is essential for proper initiation and elaboration of the blast disease in rice (PubMed:26258762). ABM, together with a polyketide synthase MGG_04775 and the esterase MGG_04774, share the secondary metabolism gene cluster with ABC transporter ABC3, and therefore may also be involved in the synthesis of other important metabolites such as the ABC3 transporter efflux substrate (ATS) and/or additional polyketides (Probable). {ECO:0000269|PubMed:26258762, ECO:0000305|PubMed:26258762}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1) |
| Keywords | Endoplasmic reticulum;Oxidoreductase;Reference proteome;Secreted;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000269|PubMed:26258762}. Secreted {ECO:0000269|PubMed:26258762}. Note=Associates with the cortical endoplasmic reticulum (ER) network during pre-invasive pathogenic development on the host surface pathogenesis and remains associated with the cortical ER in the invasive hyphae untill the later stages of host colonization (PubMed:26258762). Associates also, albeit weakly, with the biotrophic interfacial complex (BIC), a specialized secretory organelle present on the invasive hyphae in M.oryzae (PubMed:26258762). Secreted specifically during invasive growth (PubMed:26258762). {ECO:0000269|PubMed:26258762}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 24,042 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:49984; RHEA:49985 |
| Cross Reference Brenda |