Detail Information for IndEnz0010000368
IED ID IndEnz0010000368
Enzyme Type ID esterase000368
Protein Name Probable cutinase 2
EC 3.1.1.74
Cutin hydrolase 2
Gene Name ATEG_07810
Organism Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus terreus Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Enzyme Sequence MNYKLLTLALASLAAANPIERQRKIHPAVLSGGDELRNGDCQPVTFIFARASTEQGLLGGSTGPALCNRLKSALDGVACQGVGPKYQATLAANALPKGTSDEAIEEAQSLFQLAAEKCPDTQIVAGGYSQGTAVMHGAIPGLDDALKDKIKGVVLFGDTRNQQDNGQIPDFPKDKIKIYCAAGDMVCYGTLIVAAPHFSYIADVPDAADFLVSKLE
Enzyme Length 216
Uniprot Accession Number Q0CES4
Absorption
Active Site ACT_SITE 129; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P00590; ACT_SITE 184; /evidence=ECO:0000250|UniProtKB:P00590; ACT_SITE 197; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:P00590
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Cutin + H(2)O = cutin monomers.; EC=3.1.1.74; Evidence={ECO:0000255|PROSITE-ProRule:PRU10108, ECO:0000255|PROSITE-ProRule:PRU10109};
DNA Binding
EC Number 3.1.1.74
Enzyme Function FUNCTION: Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants (By similarity). Degrades cutin, a macromolecule that forms the structure of the plant cuticle (By similarity). {ECO:0000250|UniProtKB:P00590}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (3); Signal peptide (1); Site (2)
Keywords Disulfide bond;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P11373}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..16; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 22,680
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda