| IED ID | IndEnz0010000382 |
| Enzyme Type ID | esterase000382 |
| Protein Name |
11-beta-hydroxysteroid dehydrogenase 1 11-DH 11-beta-HSD1 EC 1.1.1.146 7-oxosteroid reductase EC 1.1.1.201 Corticosteroid 11-beta-dehydrogenase isozyme 1 Short chain dehydrogenase/reductase family 26C member 1 |
| Gene Name | HSD11B1 HSD11 HSD11L SDR26C1 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MAFMKKYLLPILGLFMAYYYYSANEEFRPEMLQGKKVIVTGASKGIGREMAYHLAKMGAHVVVTARSKETLQKVVSHCLELGAASAHYIAGTMEDMTFAEQFVAQAGKLMGGLDMLILNHITNTSLNLFHDDIHHVRKSMEVNFLSYVVLTVAALPMLKQSNGSIVVVSSLAGKVAYPMVAAYSASKFALDGFFSSIRKEYSVSRVNVSITLCVLGLIDTETAMKAVSGIVHMQAAPKEECALEIIKGGALRQEEVYYDSSLWTTLLIRNPCRKILEFLYSTSYNMDRFINK |
| Enzyme Length | 292 |
| Uniprot Accession Number | P28845 |
| Absorption | |
| Active Site | ACT_SITE 183; /note=Proton acceptor |
| Activity Regulation | ACTIVITY REGULATION: Hexose-6-phosphate dehydrogenase (H6PD) provides cosubstrate NADPH, and the glucose-6-phosphate transporter in the ER-membrane supplies the substrate for H6PDH, their activities stimulate the reduction of cortisone and abolish the oxidation of cortisol. {ECO:0000269|PubMed:15280030, ECO:0000269|PubMed:17593962}. |
| Binding Site | BINDING 170; /note="Substrate"; /evidence="ECO:0000269|PubMed:15513927, ECO:0007744|PDB:1XU7, ECO:0007744|PDB:1XU9" |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=an 11beta-hydroxysteroid + NADP(+) = an 11-oxosteroid + H(+) + NADPH; Xref=Rhea:RHEA:11388, ChEBI:CHEBI:15378, ChEBI:CHEBI:35346, ChEBI:CHEBI:47787, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.146; Evidence={ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:11481269, ECO:0000269|PubMed:12460758, ECO:0000269|PubMed:14973125, ECO:0000269|PubMed:15152005, ECO:0000269|PubMed:15280030, ECO:0000269|PubMed:17070044, ECO:0000269|PubMed:17593962, ECO:0000269|PubMed:17919905, ECO:0000269|PubMed:21453287, ECO:0000269|PubMed:27927697, ECO:0000269|PubMed:30902677, ECO:0000269|PubMed:8319583};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11389; Evidence={ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:12460758, ECO:0000269|PubMed:14973125, ECO:0000269|PubMed:15152005, ECO:0000269|PubMed:21453287, ECO:0000269|PubMed:30902677, ECO:0000269|PubMed:8319583, ECO:0000305|PubMed:15280030, ECO:0000305|PubMed:17593962, ECO:0000305|PubMed:27927697};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11390; Evidence={ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:11481269, ECO:0000269|PubMed:12460758, ECO:0000269|PubMed:14973125, ECO:0000269|PubMed:15152005, ECO:0000269|PubMed:21453287, ECO:0000269|PubMed:30902677, ECO:0000269|PubMed:8319583, ECO:0000305|PubMed:15280030, ECO:0000305|PubMed:17593962, ECO:0000305|PubMed:27927697}; CATALYTIC ACTIVITY: Reaction=cortisone + H(+) + NADPH = cortisol + NADP(+); Xref=Rhea:RHEA:68616, ChEBI:CHEBI:15378, ChEBI:CHEBI:16962, ChEBI:CHEBI:17650, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; Evidence={ECO:0000269|PubMed:11481269, ECO:0000269|PubMed:12460758, ECO:0000269|PubMed:15152005, ECO:0000269|PubMed:15280030, ECO:0000269|PubMed:17593962, ECO:0000269|PubMed:21453287, ECO:0000269|PubMed:27927697, ECO:0000269|PubMed:30902677, ECO:0000269|PubMed:8319583};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68617; Evidence={ECO:0000269|PubMed:11481269, ECO:0000269|PubMed:12460758, ECO:0000269|PubMed:15152005, ECO:0000269|PubMed:21453287, ECO:0000269|PubMed:30902677, ECO:0000269|PubMed:8319583, ECO:0000305|PubMed:15280030, ECO:0000305|PubMed:17593962, ECO:0000305|PubMed:27927697};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:68618; Evidence={ECO:0000269|PubMed:12460758, ECO:0000269|PubMed:15152005, ECO:0000269|PubMed:21453287, ECO:0000269|PubMed:30902677, ECO:0000269|PubMed:8319583, ECO:0000305|PubMed:15280030, ECO:0000305|PubMed:17593962, ECO:0000305|PubMed:27927697}; CATALYTIC ACTIVITY: Reaction=corticosterone + NADP(+) = 11-dehydrocorticosterone + H(+) + NADPH; Xref=Rhea:RHEA:42200, ChEBI:CHEBI:15378, ChEBI:CHEBI:16827, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78600; Evidence={ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:14973125};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42201; Evidence={ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:14973125};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:42202; Evidence={ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:14973125}; CATALYTIC ACTIVITY: Reaction=a 7beta-hydroxysteroid + NADP(+) = a 7-oxosteroid + H(+) + NADPH; Xref=Rhea:RHEA:20233, ChEBI:CHEBI:15378, ChEBI:CHEBI:35349, ChEBI:CHEBI:47789, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.201; Evidence={ECO:0000269|PubMed:14973125, ECO:0000269|PubMed:15095019, ECO:0000269|PubMed:15152005, ECO:0000269|PubMed:17593962, ECO:0000269|PubMed:21453287};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:20235; Evidence={ECO:0000269|PubMed:14973125, ECO:0000269|PubMed:15095019, ECO:0000269|PubMed:15152005, ECO:0000269|PubMed:21453287, ECO:0000305|PubMed:17593962}; CATALYTIC ACTIVITY: Reaction=7-oxocholesterol + H(+) + NADPH = 7beta-hydroxycholesterol + NADP(+); Xref=Rhea:RHEA:68656, ChEBI:CHEBI:15378, ChEBI:CHEBI:42989, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:64294; Evidence={ECO:0000269|PubMed:14973125, ECO:0000269|PubMed:15095019, ECO:0000269|PubMed:15152005};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:68657; Evidence={ECO:0000269|PubMed:14973125, ECO:0000269|PubMed:15095019, ECO:0000269|PubMed:15152005}; CATALYTIC ACTIVITY: Reaction=chenodeoxycholate + NADP(+) = 7-oxolithocholate + H(+) + NADPH; Xref=Rhea:RHEA:53820, ChEBI:CHEBI:15378, ChEBI:CHEBI:36234, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78605; Evidence={ECO:0000269|PubMed:21453287};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:53822; Evidence={ECO:0000269|PubMed:21453287}; CATALYTIC ACTIVITY: Reaction=7-oxolithocholate + H(+) + NADPH = NADP(+) + ursodeoxycholate; Xref=Rhea:RHEA:47540, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78604, ChEBI:CHEBI:78605; Evidence={ECO:0000269|PubMed:21453287};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47541; Evidence={ECO:0000269|PubMed:21453287}; CATALYTIC ACTIVITY: Reaction=glycochenodeoxycholate + NADP(+) = 7-oxoglycolithocholate + H(+) + NADPH; Xref=Rhea:RHEA:65056, ChEBI:CHEBI:15378, ChEBI:CHEBI:36252, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:137818; Evidence={ECO:0000269|PubMed:21453287};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:65058; Evidence={ECO:0000269|PubMed:21453287}; CATALYTIC ACTIVITY: Reaction=NADP(+) + taurochenodeoxycholate = 7-oxotaurolithocholate + H(+) + NADPH; Xref=Rhea:RHEA:65060, ChEBI:CHEBI:9407, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:137724; Evidence={ECO:0000269|PubMed:21453287};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:65062; Evidence={ECO:0000269|PubMed:21453287}; CATALYTIC ACTIVITY: Reaction=NADP(+) + tauroursodeoxycholate = 7-oxotaurolithocholate + H(+) + NADPH; Xref=Rhea:RHEA:68980, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132028, ChEBI:CHEBI:137724; Evidence={ECO:0000269|PubMed:21453287};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:68982; Evidence={ECO:0000269|PubMed:21453287}; CATALYTIC ACTIVITY: Reaction=glycoursodeoxycholate + NADP(+) = 7-oxoglycolithocholate + H(+) + NADPH; Xref=Rhea:RHEA:68976, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132030, ChEBI:CHEBI:137818; Evidence={ECO:0000269|PubMed:21453287};PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:68978; Evidence={ECO:0000269|PubMed:21453287}; CATALYTIC ACTIVITY: Reaction=7-oxopregnenolone + H(+) + NADPH = 7beta-hydroxypregnenolone + NADP(+); Xref=Rhea:RHEA:69436, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:183806, ChEBI:CHEBI:183807; Evidence={ECO:0000269|PubMed:17593962};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69437; Evidence={ECO:0000305|PubMed:17593962}; CATALYTIC ACTIVITY: Reaction=3beta,7alpha-dihydroxyandrost-5-en-17-one + NADP(+) = 3beta-hydroxy-5-androstene-7,17-dione + H(+) + NADPH; Xref=Rhea:RHEA:69440, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:81471, ChEBI:CHEBI:183808; Evidence={ECO:0000269|PubMed:17593962};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69441; Evidence={ECO:0000305|PubMed:17593962}; CATALYTIC ACTIVITY: Reaction=3beta-hydroxy-5-androstene-7,17-dione + H(+) + NADPH = 3beta,7beta-dihydroxyandrost-5-en-17-one + NADP(+); Xref=Rhea:RHEA:69452, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:183368, ChEBI:CHEBI:183808; Evidence={ECO:0000269|PubMed:17593962};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69453; Evidence={ECO:0000305|PubMed:17593962}; CATALYTIC ACTIVITY: Reaction=3beta-hydroxy-5alpha-androstane-7,17-dione + H(+) + NADPH = 3beta,7beta-dihydroxy-5alpha-androstan-17-one + NADP(+); Xref=Rhea:RHEA:69456, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:79834, ChEBI:CHEBI:183809; Evidence={ECO:0000269|PubMed:17593962};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:69457; Evidence={ECO:0000305|PubMed:17593962}; |
| DNA Binding | |
| EC Number | 1.1.1.146; 1.1.1.201 |
| Enzyme Function | FUNCTION: Controls the reversible conversion of biologically active glucocorticoids such as cortisone to cortisol, and 11-dehydrocorticosterone to corticosterone in the presence of NADP(H) (PubMed:10497248, PubMed:12460758, PubMed:14973125, PubMed:15152005, PubMed:15280030, PubMed:17593962, PubMed:21453287, PubMed:27927697, PubMed:30902677). Participates in the corticosteroid receptor-mediated anti-inflammatory response, as well as metabolic and homeostatic processes (PubMed:12414862, PubMed:10497248, PubMed:15152005, PubMed:21453287). Plays a role in the secretion of aqueous humor in the eye, maintaining a normotensive, intraocular environment (PubMed:11481269). Bidirectional in vitro, predominantly functions as a reductase in vivo, thereby increasing the concentration of active glucocorticoids (PubMed:12414862, PubMed:10497248, PubMed:11481269, PubMed:12460758). It has broad substrate specificity, besides glucocorticoids, it accepts other steroid and sterol substrates (PubMed:15095019, PubMed:15152005, PubMed:17593962, PubMed:21453287). Interconverts 7-oxo- and 7-hydroxy-neurosteroids such as 7-oxopregnenolone and 7beta-hydroxypregnenolone, 7-oxodehydroepiandrosterone (3beta-hydroxy-5-androstene-7,17-dione) and 7beta-hydroxydehydroepiandrosterone (3beta,7beta-dihydroxyandrost-5-en-17-one), among others (PubMed:17593962). Catalyzes the stereo-specific conversion of the major dietary oxysterol, 7-ketocholesterol (7-oxocholesterol), into the more polar 7-beta-hydroxycholesterol metabolite (PubMed:15095019, PubMed:15152005). 7-oxocholesterol is one of the most important oxysterols, it participates in several events such as induction of apoptosis, accumulation in atherosclerotic lesions, lipid peroxidation, and induction of foam cell formation (PubMed:15095019). Mediates the 7-oxo reduction of 7-oxolithocholate mainly to chenodeoxycholate, and to a lesser extent to ursodeoxycholate, both in its free form and when conjugated to glycine or taurine, providing a link between glucocorticoid activation and bile acid metabolism (PubMed:21453287). Catalyzes the synthesis of 7-beta-25-dihydroxycholesterol from 7-oxo-25-hydroxycholesterol in vitro, which acts as ligand for the G-protein-coupled receptor (GPCR) Epstein-Barr virus-induced gene 2 (EBI2) and may thereby regulate immune cell migration (PubMed:30902677). {ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:12460758, ECO:0000269|PubMed:14973125, ECO:0000269|PubMed:15095019, ECO:0000269|PubMed:15152005, ECO:0000269|PubMed:17593962, ECO:0000269|PubMed:21453287, ECO:0000269|PubMed:27927697, ECO:0000269|PubMed:30902677, ECO:0000303|PubMed:10497248, ECO:0000303|PubMed:11481269, ECO:0000303|PubMed:12414862, ECO:0000303|PubMed:12460758, ECO:0000303|PubMed:15095019, ECO:0000303|PubMed:15152005, ECO:0000303|PubMed:21453287}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Steroid metabolism. {ECO:0000305}. |
| nucleotide Binding | NP_BIND 41..67; /note="NADP"; /evidence="ECO:0000269|PubMed:15513927, ECO:0000269|PubMed:17919905, ECO:0000269|PubMed:18069989, ECO:0000269|PubMed:18485702, ECO:0000269|PubMed:18553955, ECO:0000269|PubMed:19217779"; NP_BIND 92..93; /note="NADP"; /evidence="ECO:0000269|PubMed:15513927, ECO:0000269|PubMed:17919905, ECO:0000269|PubMed:18069989, ECO:0000269|PubMed:18485702, ECO:0000269|PubMed:18553955, ECO:0000269|PubMed:19217779"; NP_BIND 119..121; /note="NADP"; /evidence="ECO:0000269|PubMed:15513927, ECO:0000269|PubMed:17919905, ECO:0000269|PubMed:18069989, ECO:0000269|PubMed:18485702, ECO:0000269|PubMed:18553955, ECO:0000269|PubMed:19217779"; NP_BIND 183..187; /note="NADP"; /evidence="ECO:0000269|PubMed:15513927, ECO:0000269|PubMed:17919905, ECO:0000269|PubMed:18069989, ECO:0000269|PubMed:18485702, ECO:0000269|PubMed:18553955, ECO:0000269|PubMed:19217779"; NP_BIND 218..222; /note="NADP"; /evidence="ECO:0000269|PubMed:15513927, ECO:0000269|PubMed:17919905, ECO:0000269|PubMed:18069989, ECO:0000269|PubMed:18485702, ECO:0000269|PubMed:18553955, ECO:0000269|PubMed:19217779" |
| Features | Active site (1); Beta strand (8); Binding site (1); Chain (1); Glycosylation (3); Helix (15); Mutagenesis (15); Natural variant (1); Nucleotide binding (5); Topological domain (2); Transmembrane (1) |
| Keywords | 3D-structure;Disease variant;Endoplasmic reticulum;Glycoprotein;Lipid metabolism;Membrane;NADP;Oxidoreductase;Reference proteome;Signal-anchor;Steroid metabolism;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:15280030}; Single-pass type II membrane protein {ECO:0000269|PubMed:10497248}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:19159218}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (40) |
| Cross Reference PDB | 1XU7; 1XU9; 2BEL; 2ILT; 2IRW; 2RBE; 3BYZ; 3BZU; 3CH6; 3CZR; 3D3E; 3D4N; 3D5Q; 3EY4; 3FCO; 3FRJ; 3H6K; 3HFG; 3OQ1; 3PDJ; 3QQP; 3TFQ; 4BB5; 4BB6; 4C7J; 4C7K; 4HFR; 4HX5; 4IJU; 4IJV; 4IJW; 4K1L; 4P38; 4YYZ; 5PGU; 5PGV; 5PGW; 5PGX; 5PGY; 5QII; |
| Mapped Pubmed ID | 11114699; 11755176; 11956655; 12109593; 12202416; 12390875; 12530648; 12530692; 12788882; 12861241; 12915682; 12915696; 12960005; 14557491; 14583441; 14682470; 14742837; 15156315; 15167446; 15172126; 15181046; 15452033; 15455200; 15536220; 15591536; 15827106; 15855321; 15941907; 16091483; 16236446; 16343739; 16368752; 16406280; 16412558; 16469798; 16551740; 16580270; 16603347; 16622297; 16855188; 16914598; 16996097; 17032748; 17062770; 17110106; 17306259; 17495191; 17519316; 17588937; 17766714; 17825335; 17912154; 17922306; 18031327; 18261751; 18313835; 18379559; 18388900; 18419108; 18434359; 18511278; 18573267; 18592327; 18611262; 18633104; 18665910; 18716005; 18769825; 18778749; 18789704; 18818748; 18837961; 19010388; 19019335; 19022342; 19022912; 19088256; 19121282; 19129713; 19158196; 19167490; 19185488; 19235128; 19251840; 19282465; 19336370; 19429429; 19453261; 19491387; 19527514; 19535162; 19540999; 19542242; 19549748; 19564704; 19567539; 19571556; 19598235; 19651257; 19673466; 19675138; 19690925; 19806478; 19876004; 19880789; 19913121; 19934376; 20041117; 20045052; 20100573; 20140262; 20214802; 20416077; 20486282; 20514603; 20628086; 20739946; 20800085; 20826586; 20971000; 21093258; 21111044; 21147223; 21189603; 21215738; 21249616; 21266326; 21304964; 21325058; 21362233; 21402901; 21439819; 21544007; 21622477; 21695543; 21704348; 21736538; 21825133; 21869537; 21983444; 21988832; 22112815; 22136330; 22179810; 22207732; 22238371; 22310475; 22357964; 22384521; 22445027; 22691057; 22719058; 22986073; 23009206; 23017470; 23088558; 23090578; 23153367; 23190557; 23362866; 23375992; 23478147; 23612224; 23659736; 23678035; 23701286; 23722901; 23778418; 23836520; 23845218; 23869418; 23874920; 23979790; 24054540; 24055708; 24061267; 24086653; 24122936; 24147632; 24243637; 24285685; 24391882; 24422550; 24447082; 24729284; 24810847; 24915124; 24969481; 25050169; 25393644; 25436731; 25659145; 25666420; 25751397; 25788665; 25989394; 26163245; 26212138; 26452272; 26528718; 26671915; 26934392; 27083553; 27219880; 27268236; 27507896; 27533889; 27544778; 27697223; 27698011; 27715400; 27721188; 28237720; 28537398; 28619249; 28698139; 28750217; 28765040; 28793178; 2889032; 28994390; 29078846; 29474943; 30445609; 30613321; 30678666; 30796905; 30825506; 31039398; 31704233; 31902257; 31922957; 32336752; 32360870; 32453474; 32880710; 32945429; 33214595; 33639236; 33722534; 34072239; 34639470; 34845968; |
| Motif | |
| Gene Encoded By | |
| Mass | 32,401 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=163 nM for corticosterone {ECO:0000269|PubMed:10497248}; KM=594 nM for 11-dehydrocorticosterone {ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:14973125}; KM=165 nM for corticosterone {ECO:0000269|PubMed:14973125}; KM=564 nM for 7-oxocholesterol {ECO:0000269|PubMed:14973125}; KM=0.140 uM for cortisone {ECO:0000269|PubMed:8319583}; KM=1.66 uM for cortisol {ECO:0000269|PubMed:8319583}; KM=0.6 uM for cortisone {ECO:0000269|PubMed:12460758}; KM=1.7 uM for cortisol {ECO:0000269|PubMed:12460758}; KM=803 nM for cortisone {ECO:0000269|PubMed:15152005}; KM=665 nM for cortisone {ECO:0000269|PubMed:15280030}; KM=1093 nM for cortisol {ECO:0000269|PubMed:15280030}; KM=826 nM for cortisol {ECO:0000269|PubMed:15152005}; KM=980 nM for 7-oxolithocholate {ECO:0000269|PubMed:21453287}; Vmax=0.42 nmol/min/mg enzyme with corticosterone as substrate {ECO:0000269|PubMed:10497248}; Vmax=0.51 nmol/min/mg enzyme with 11-dehydrocorticosterone as substrate {ECO:0000269|PubMed:10497248, ECO:0000269|PubMed:14973125}; Vmax=0.45 nmol/min/mg enzyme with corticosterone as substrate {ECO:0000269|PubMed:14973125}; Vmax=0.21 nmol/min/mg enzyme with 7-oxocholesterol as substrate {ECO:0000269|PubMed:14973125}; Vmax=540 nmol/h/ug enzyme with cortisol as substrate {ECO:0000269|PubMed:8319583}; Vmax=0.216 nmol/min/mg enzyme with cortisone as substrate {ECO:0000269|PubMed:8319583}; Vmax=0.76 nmol/h/mg enzyme with cortisone as substrate {ECO:0000269|PubMed:15280030}; Vmax=3.8 nmol/min/mg enzyme with cortisone as substrate {ECO:0000269|PubMed:12460758}; Vmax=1.74 nmol/h/mg enzyme with cortisol as substrate {ECO:0000269|PubMed:15280030}; Vmax=69.8 nmol/min/mg enzyme with cortisol as substrate {ECO:0000269|PubMed:12460758}; Vmax=0.72 nmol/h/mg enzyme with cortisone as substrate {ECO:0000269|PubMed:15152005}; Vmax=1.59 nmol/h/mg enzyme with cortisone as substrate {ECO:0000269|PubMed:30902677}; Vmax=0.64 nmol/h/mg enzyme with cortisol as substrate {ECO:0000269|PubMed:15152005}; Vmax=2.8 nmol/h/mg enzyme with 7-oxolithocholate as substrate {ECO:0000269|PubMed:21453287}; |
| Metal Binding | |
| Rhea ID | RHEA:11388; RHEA:11389; RHEA:11390; RHEA:68616; RHEA:68617; RHEA:68618; RHEA:42200; RHEA:42201; RHEA:42202; RHEA:20233; RHEA:20235; RHEA:68656; RHEA:68657; RHEA:53820; RHEA:53822; RHEA:47540; RHEA:47541; RHEA:65056; RHEA:65058; RHEA:65060; RHEA:65062; RHEA:68980; RHEA:68982; RHEA:68976; RHEA:68978; RHEA:69436; RHEA:69437; RHEA:69440; RHEA:69441; RHEA:69452; RHEA:69453; RHEA:69456; RHEA:69457 |
| Cross Reference Brenda | 1.1.1.146;1.1.1.B40; |