| IED ID | IndEnz0010000384 |
| Enzyme Type ID | esterase000384 |
| Protein Name |
Acetylxylan esterase / glucomannan deacetylase EC 3.1.1.- EC 3.1.1.72 CjCE2A |
| Gene Name | axe2C CJA_0450 |
| Organism | Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Cellvibrionales Cellvibrionaceae Cellvibrio Cellvibrio japonicus (Pseudomonas fluorescens subsp. cellulosa) Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) |
| Enzyme Sequence | MKLLFPILLLTGSYFLSACNNTQSLMSSTHTIAASDPHIQVMGRTHINDDASLTFGYPGVSLSTIVAGSRLTAEMQSSNGNSWIDVIIDNHPPTSIKLDAQQQTVELFHFPNSGEHRVEIIHRSENWHGQVTLKQLTLTGTQFLPAPVLPQRKILVLGDSVTCGEAIDRVAGEDKNTRWWNARESYGMLTAKALDAQVQLVCWGGRGLIRSWNGKTDDANLPDFYQFTLGDTGQAPQWDHHRYQPDLIISAIGTNDFSPGIPDRATYINTYTRFVRTLLDNHPQATIVLTEGAILNGDKKAALVSYIGETRQQLHSNRVFYASSSHHPGDNSDAHPTKDQHAAMARELTPQLRQIMDW |
| Enzyme Length | 358 |
| Uniprot Accession Number | B3PIB0 |
| Absorption | |
| Active Site | ACT_SITE 160; /note=Nucleophile; /evidence=ECO:0000305|PubMed:19338387; ACT_SITE 333; /note=Charge relay system; /evidence=ECO:0000305|PubMed:19338387; ACT_SITE 335; /note=Charge relay system; /evidence=ECO:0000305|PubMed:19338387 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Deacetylation of xylans and xylo-oligosaccharides.; EC=3.1.1.72; Evidence={ECO:0000269|PubMed:19338387}; |
| DNA Binding | |
| EC Number | 3.1.1.-; 3.1.1.72 |
| Enzyme Function | FUNCTION: Involved in the degradation of plant cell wall polysaccharides. Catalyzes the deacetylation of acetylated birchwood xylan and glucomannan, with equal efficiency, and of the synthetic substrate 4-nitrophenyl acetate (4-NPAc). Does not bind cellulose, cellohexaose and beta-glucan. {ECO:0000269|PubMed:19338387}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. {ECO:0000269|PubMed:19338387}. |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (17); Chain (1); Helix (9); Lipidation (2); Signal peptide (1); Site (2); Turn (1) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cell membrane;Glycosidase;Hydrolase;Lipoprotein;Membrane;Palmitate;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255|PROSITE-ProRule:PRU00303 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 2WAA; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,779 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=339 uM for 4-nitrophenyl acetate {ECO:0000269|PubMed:19338387}; KM=1.5 mM for acetylated birchwood xylan {ECO:0000269|PubMed:19338387}; KM=4.2 mM for acetylated glucomannan {ECO:0000269|PubMed:19338387}; Note=kcat is 7717 min(-1) for the deacetylation of 4-nitrophenyl acetate. kcat is 69 min(-1) for the deacetylation of birchwood xylan. kcat is 163 min(-1) for the deacetylation of glucomannan. {ECO:0000269|PubMed:19338387}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |