| IED ID | IndEnz0010000385 |
| Enzyme Type ID | esterase000385 |
| Protein Name |
Probable cutinase 3 EC 3.1.1.74 Cutin hydrolase 3 |
| Gene Name | AO090011000113 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MVMLRSLLVSALAALAAGSPIAEPADQSLEARQLGSSNDLTNGACKDVTLIFARGSTEMGNMGTVIGPPLCSSLKSKLGADKVACQGVGGLYTGGLMQNALPQNTDPGAISTAKSLFEQASTKCPNTQIVAGGYSQGSAVIDNAVQQLSAEVKDKVKGVVFFGFTRNLQDKGQIPNYPKDNVKVFCAMGDLVCDGTLIVTAAHLTYTINAPEAASFLASKVQSA |
| Enzyme Length | 224 |
| Uniprot Accession Number | Q2U199 |
| Absorption | |
| Active Site | ACT_SITE 135; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P00590; ACT_SITE 190; /evidence=ECO:0000250|UniProtKB:P00590; ACT_SITE 203; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:P00590 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cutin + H(2)O = cutin monomers.; EC=3.1.1.74; Evidence={ECO:0000255|PROSITE-ProRule:PRU10108, ECO:0000255|PROSITE-ProRule:PRU10109}; |
| DNA Binding | |
| EC Number | 3.1.1.74 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants (By similarity). Degrades cutin, a macromolecule that forms the structure of the plant cuticle (By similarity). {ECO:0000250|UniProtKB:P00590}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Signal peptide (1); Site (2) |
| Keywords | Disulfide bond;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P11373}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,075 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |