| IED ID | IndEnz0010000392 |
| Enzyme Type ID | esterase000392 |
| Protein Name |
Acetylxylan esterase AXE EC 3.1.1.72 |
| Gene Name | CC1G_12850 |
| Organism | Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Psathyrellaceae Coprinopsis Coprinopsis cinerea (Inky cap fungus) (Hormographiella aspergillata) Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata) |
| Enzyme Sequence | MVFSPRLSAFVALVALTNAATAVPMYGQCGGSGYTGPTQCDPGLVCVKLNDWYSQCQSGGAQPPVTTTSSPPVTVSPPPSTTTVAPPVATGPPAPEIPAGQLTQLRSFGNNPSNISMFVYKPQNVKNRPGLLVALHPCGGTAQQYFSGFPGFRQHADQRGFIVLYGQSPPGSSNCWDIISTASLTREGGDDSTGIASAVKYALQNWNVDPEKVFVTGTSSGAMMTNIMAATYPDLFKAGAVWAGTAVGCLSANTPQFPPDPCQSGTVIRTPQEWGDRVRRAYPGYNGPWPRMQIWHGTNDFALDHKNLAEQMKQWTNVHNISQTPTSTSPSTPRQGWTKQVYGNGLVETFSGQGAGHGLPESGTEVVAMDFFGL |
| Enzyme Length | 374 |
| Uniprot Accession Number | A8PB24 |
| Absorption | |
| Active Site | ACT_SITE 219; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Deacetylation of xylans and xylo-oligosaccharides.; EC=3.1.1.72; Evidence={ECO:0000269|PubMed:23180549}; |
| DNA Binding | |
| EC Number | 3.1.1.72 |
| Enzyme Function | FUNCTION: Acetylxylan esterase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Degrades acetylated xylans by cleaving acetyl side groups from the hetero-xylan backbone. {ECO:0000269|PubMed:23180549}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 40 degrees Celsius. {ECO:0000269|PubMed:23180549}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8. {ECO:0000269|PubMed:23180549}; |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. {ECO:0000305|PubMed:23180549}. |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Domain (1); Glycosylation (2); Region (3); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Cellulose degradation;Glycoprotein;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Serine esterase;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:23180549}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:23180549}. |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,665 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4.3 mM for 4-nitrophanyl acetate {ECO:0000269|PubMed:23180549}; KM=0.11 mM for 4-nitrophanyl butyrate {ECO:0000269|PubMed:23180549}; Vmax=2.15 umol/min/mg enzyme 4-nitrophanyl acetate {ECO:0000269|PubMed:23180549}; Vmax=0.78 umol/min/mg enzyme 4-nitrophanyl butyrate {ECO:0000269|PubMed:23180549}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |