| IED ID | IndEnz0010000395 |
| Enzyme Type ID | esterase000395 |
| Protein Name |
Acetylxylan esterase 2 EC 3.1.1.72 AXE II |
| Gene Name | axe-2 axeII |
| Organism | Talaromyces purpureogenus (Soft rot fungus) (Penicillium purpureogenum) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Trichocomaceae Talaromyces Talaromyces sect. Talaromyces Talaromyces purpureogenus (Soft rot fungus) (Penicillium purpureogenum) |
| Enzyme Sequence | MHSKFFAASLLGLGAAAIPLEGVMEKRSCPAIHVFGARETTASPGYGSSSTVVNGVLSAYPGSTAEAINYPACGGQSSCGGASYSSSVAQGIAAVASAVNSFNSQCPSTKIVLVGYSQGGEIMDVALCGGGDPNQGYTNTAVQLSSSAVNMVKAAIFMGDPMFRAGLSYEVGTCAAGGFDQRPAGFSCPSAAKIKSYCDASDPYCCNGSNAATHQGYGSEYGSQALAFVKSKLG |
| Enzyme Length | 234 |
| Uniprot Accession Number | O59893 |
| Absorption | |
| Active Site | ACT_SITE 117; /evidence=ECO:0000250; ACT_SITE 202; /evidence=ECO:0000250; ACT_SITE 214; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Deacetylation of xylans and xylo-oligosaccharides.; EC=3.1.1.72; |
| DNA Binding | |
| EC Number | 3.1.1.72 |
| Enzyme Function | FUNCTION: Degrades acetylated xylans by cleaving acetyl side groups from the hetero-xylan backbone. {ECO:0000269|PubMed:8756392}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius. {ECO:0000269|PubMed:8756392}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6. {ECO:0000269|PubMed:8756392}; |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (7); Chain (1); Disulfide bond (5); Glycosylation (1); Helix (10); Propeptide (1); Sequence conflict (1); Signal peptide (1); Turn (1) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Polysaccharide degradation;Secreted;Serine esterase;Signal |
| Interact With | |
| Induction | INDUCTION: Induced by xylan and repressed by glucose. Expressed at neutral pH, but not under alkaline or acidic condtions. {ECO:0000269|PubMed:15174310}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8756392}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 1BS9; 1G66; 2AXE; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,478 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |