| IED ID | IndEnz0010000397 |
| Enzyme Type ID | esterase000397 |
| Protein Name |
N-acetylneuraminate 9-O-acetyltransferase EC 2.3.1.45 CAS1 domain-containing protein 1 Sialate O-acetyltransferase SOAT |
| Gene Name | Casd1 Cas1 Cast1 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MAALAYNLGKREINHYFSVRSAKVLALVAVLLLAACHLASRRYRGNDSCEYLLSSGRFLGEKVWQPHSCMMHKYKISEAKTCLVDKHIAFIGDSRIRQLFYSFVKIINPQFKEEGNKHENIPFEDKAASVKVDFLWHPEVNGSMKQCIKVWTEDSVLKPHVIVAGAATWSIKIHNGSEEALAQYKMNITSIAPLLEKLAKTSDVYWVLQDPVYEDLLSENRKMITNEKIDAYNEAAVSILNSSTRTSKSNVKMFSVSKLIAQETIMESLDGLHLPESSRETSAMILMNVYCNKVVKPVDGSCCQPRPPLTLIQKLAACFFTLSIIGYFIFYVIHRNAHRKNKPCTDLESGEEKKNIINTPVSSLEILLQSFCKLGLIMAYFYMCDRANLFMKENKFYTHSSFFIPIIYILVLGVFYNENTKETKVLNREQTDEWKGWMQLVILIYHISGASTFLPVYMHIRVLVAAYLFQTGYGHFSYFWIKGDFGIHRVCQVLFRLNFLVVVLCIVMDRPYQFYYFVPLVTVWFMVIYVTLALWPQITQKKANGNFFWYLGLLLKLGLLLLCIWFLAYSQGAFEKIFSLWPLSKCFELEGSVYEWWFRWRLDRYVVFHGVLFAFIYLALQRRQILSEGKGEPLFSNKISNFLLFVSVVSFLTYSIWASSCKNKAECNELHPSVSVVQIVAFILIRNIPGYARSIYSSFFAWFGKISLELFICQYHIWLAADTRGILVLIPGNPTLNIIVSTFIFVCVAHEISQITTDLAQVVIPKDNPSLFRRLACTIAFFGGVLILSSIQDKSRL |
| Enzyme Length | 797 |
| Uniprot Accession Number | Q7TN73 |
| Absorption | |
| Active Site | ACT_SITE 94; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q96PB1; ACT_SITE 270; /evidence=ECO:0000250|UniProtKB:Q96PB1; ACT_SITE 273; /evidence=ECO:0000250|UniProtKB:Q96PB1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=acetyl-CoA + N-acetylneuraminate = CoA + N-acetyl-9-O-acetylneuraminate; Xref=Rhea:RHEA:26043, ChEBI:CHEBI:28999, ChEBI:CHEBI:35418, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.45; Evidence={ECO:0000250|UniProtKB:Q96PB1}; CATALYTIC ACTIVITY: Reaction=acetyl-CoA + N-acetylneuraminate = CoA + N-acetyl-7-O-acetylneuraminate; Xref=Rhea:RHEA:20808, ChEBI:CHEBI:28944, ChEBI:CHEBI:35418, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.45; Evidence={ECO:0000250|UniProtKB:Q96PB1}; |
| DNA Binding | |
| EC Number | 2.3.1.45 |
| Enzyme Function | FUNCTION: O-acetyltransferase that catalyzes 9-O-acetylation of sialic acids. Sialic acids are sugars at the reducing end of glycoproteins and glycolipids, and are involved in various processes such as cell-cell interactions, host-pathogen recognition. {ECO:0000250|UniProtKB:Q96PB1}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous initiation (2); Glycosylation (3); Topological domain (16); Transmembrane (15) |
| Keywords | Acyltransferase;Glycoprotein;Golgi apparatus;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250|UniProtKB:Q96PB1}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q96PB1}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q96PB1}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14610273; 16341224; 19812333; 30597004; |
| Motif | |
| Gene Encoded By | |
| Mass | 91,603 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:26043; RHEA:20808 |
| Cross Reference Brenda |