Detail Information for IndEnz0010000403
IED ID IndEnz0010000403
Enzyme Type ID esterase000403
Protein Name Probable carboxylesterase Culp2
EC 3.1.1.-
CFP25
Cutinase-like protein 2
Culp2
Gene Name cut2 Rv2301 MTCY339.08c
Organism Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Enzyme Sequence MNDLLTRRLLTMGAAAAMLAAVLLLTPITVPAGYPGAVAPATAACPDAEVVFARGRFEPPGIGTVGNAFVSALRSKVNKNVGVYAVKYPADNQIDVGANDMSAHIQSMANSCPNTRLVPGGYSLGAAVTDVVLAVPTQMWGFTNPLPPGSDEHIAAVALFGNGSQWVGPITNFSPAYNDRTIELCHGDDPVCHPADPNTWEANWPQHLAGAYVSSGMVNQAADFVAGKLQ
Enzyme Length 230
Uniprot Accession Number P9WP41
Absorption
Active Site ACT_SITE 123; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O53581; ACT_SITE 189; /evidence=ECO:0000250|UniProtKB:O53581; ACT_SITE 207; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:O53581
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.1.1.-
Enzyme Function FUNCTION: Shows weak esterase activity with the p-nitrophenol-linked aliphatic ester pNP-butyrate. Does not exhibit cutinase activity. {ECO:0000269|PubMed:19225166}.; FUNCTION: Induces interferon-gamma (IFN-gamma) release in animal models and in human TB patients (PubMed:9673225, PubMed:10076913). Also induces a strong delayed-type hypersensitivity (DTH) response in animal models (PubMed:9673225). {ECO:0000269|PubMed:10076913, ECO:0000269|PubMed:9673225}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (2); Signal peptide (1)
Keywords Direct protein sequencing;Disulfide bond;Hydrolase;Reference proteome;Secreted;Serine esterase;Signal;Virulence
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19225166, ECO:0000269|PubMed:9673225}. Cell surface {ECO:0000269|PubMed:21594640}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..43; /evidence=ECO:0000269|PubMed:9673225
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 23,926
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda