| IED ID | IndEnz0010000433 |
| Enzyme Type ID | esterase000433 |
| Protein Name |
Lysophospholipase NTE1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | NTE1 YALI0B11044g |
| Organism | Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Dipodascaceae Yarrowia Yarrowia lipolytica (Candida lipolytica) Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) |
| Enzyme Sequence | MDSLHVSSTSVLVDVVEAVETATSLVVDTAEAVATEQATPTAVISNALARSAYAAHTSLSYLAWAFGLWFLRLIGWVCYGIPTYVLGLLGRTINISLQFSSLLLILIALVTVVVAVVRYKYLTVYSRLPQEQPRQEPEIDMYDESDNEDEKTGFANYFDEFLSAVKIFGYLERPVFHELTRHMQTWKLSADEMVPLDDEQGFSVVVEGTVQVFAKQSSFVQNPTSVPDSRKEDSIMFNGERYTLLSEIKNGAPLTSLFNILSLFTDDLQLHKNFDSAVNSPMTSASDVPNMSLSSDGSDDLQKGEPQFGEPRLSEKPAAKLSVTVRAATDSTIAIIPAAAFRRITKKFPQATAHIVQVILTRFQRVTFQTGHHYFGLTPEIFQTEVNLNSHARNELPGYLREGAVKKLNQVYDASQMGGRPKKYTVTLNKKNKGKGSRRQRFSVQLNNQGHLNSQSRMVSLDSLEAVGDHMNPGDLLTNVPLSRQGRPVFELSSVKHKASIQNLSFSGNDDENEDTALRTALVEAIFKVLGIDRDSIQSSIMAVKTMSNTASPMFTGATTGGSSGSLGEELRSRRTGQDSLGASHFGVGLPSERSQNSFYARSETSTSSVDEDSLMAAPFDTIRNDVAQYMDVVLFKKDSLLIKQDDPTPGLYYLIDGVLEVGYTDHHKIYHDLYTVQPGGVGGYIGSILGHRSFADLRARTDVYAGFLPRAAIERMSDKYPMVHLTMAKSLTKVLSRLLLHLDFAMEWVQVRAGQKIYKEGQEADAIYIVLNGRVRSVAETKGDSGIVGGESGDAKDGKSHRKNLTSIGEYGKGESVGELEVLTLTRRPSTLVAIRDAELAKIPRALFESLALHYPSITFEISRIVASRVRTLMEDSAPIPRRMHTFDMAAHHDSYLTIAVVPISQDVDVSEFGRRLYNGMQAVGREACHLNHASVLNHMGRHAFNPLGKLKLSGFLDDIEDRYQTVLYVADTPPGSSWTHTCISQADCVLLVADARSEPDIGEYERVLVKMRTTARTEMVLIHPERYVPPGLTSAWLKPRVWVHTHHHVQMDLPRHEADVLASIRKMKRTGTLANLKNKVQTIQEEFRSMYRPKANIYSTSSANKDDFNRLARILSGQAIGLVLGGGGARGISHIGIIKALEDSGIPIDFVGGTSIGSFIGGLYAKEYDLVPIYGRAKKFSGRVSSLWRMALDLTYPATSYTTGHEFNRGIWKAFGDSRIEDFWLRYFTNTTNITHSRMEIHTSGYAWRYIRASMSLAGLLPPLTDNGSMLLDGGYVDNLPVSEMKAQGASVVFAVDVGSIDDTTPMNYGDSLSGAWVMWNRWNPFGRHPNVPNLAEIQARLAYVSSVGALEKAKHTPGVIYMRPPIDDFATLDFAKFLDIYRVGNKYGHKFLTELREDGKFPAIPGMENVKTKHKRTIARRNSI |
| Enzyme Length | 1427 |
| Uniprot Accession Number | Q6CF18 |
| Absorption | |
| Active Site | ACT_SITE 1157; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1275; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 615..735; /note=cNMP 1; NP_BIND 731..870; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Compositional bias (1); Domain (1); Motif (3); Nucleotide binding (2); Region (2); Topological domain (3); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1128..1133; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1155..1159; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1275..1277; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 158,153 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |