| IED ID | IndEnz0010000435 |
| Enzyme Type ID | esterase000435 |
| Protein Name |
Lysophospholipase NTE1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | NTE1 PGUG_02654 |
| Organism | Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Debaryomycetaceae Meyerozyma Meyerozyma guilliermondii (Yeast) (Candida guilliermondii) Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii) |
| Enzyme Sequence | MDSDTSSADFHSTETLVSTPKYSYGVLINVILLVSWTCFRVVNWFLVTLPSILLGMLSKTFQITLSLSSILMFVVAVTAICFLVVRYKYLTRYSRNSAAKEPAKKSFDIDDLSKKKKSSSKTSSNYLDQFLSAIQVFGYLEGPVFHELTRNMTTQKVLPNEVLYLDESLGFSIVVEGTMQVYTKVTNLSNSSLNAEDDGLDLRYENDDVLCIGNDKYQLLNEVKSGAPLSSLMSTLDLFTSNEVPLSPTKFGSYSLTAVTPLELSPNNTGGNSPPPSLAPEIVARPKGTTSTTLAIIPYTAFQRLHKKYPKATSHIVTMVLTRLYKVSMNTIYNYLGLSKDILDCERNLNAETNERLPSYLSKGIIEKFHKTEESASVEGSPTLPRRSTVNLRALTRQASSSRYVVLASRSKSTHPGDLLSSVPLSRRSDYYETQTIPNESEDSPTIQRSSLRRRASHSTSLRKSNSVLEDIRVRGFSNEREETEETSLRTAIVEQIFTHLGISEDDPTVMNINPTSASSSASSSIVGISKLNYGSLDSINSPRVDPMTSIRSSLASNGNKVYQTINQHTKEADVLQEKPKKVVLPGSRGDDSDFVAVKDEFARAIQMKYIQPGKTIIEQDSFHTGIFYVIDGSLDVIQESNGKSKKIYTVKAGGITGYLNCLIGMKSLVSVKASEDSSAIVAHIPSHMYSKLIDKFYFLQLPIARRLKRLLSKQFLTIDYALEWCHISAGDILSKEGEPANGFHIVLSGRFRVVKFKTPFKPVASKAGHDTYDYNDNLIDESNKKYTSNDVEILGEYGHGESIGEVEVLTASLRKNTLIAVRDSETARIPRALFELLSLQNPSIMVKVSRIVANRLSKKDSEEILDKPLAMTSSDSPYISSNFKTITILPTVSQLPVREFADKLVQALKAIGRNVIALDQASTLTHLGKHAFDERLAELKLSGYFAYLEEKYETIVYVCDTPVKSNWTSTCISQGDCILLLADADDEDTAESIGSYERLLIKLKTTARTDLCLIHPEKYVTPGSTSIWLKNRVWVQGHHHIQMEISPTQTTSVMKPKIPIISELAKKIKDRANPNIKLKLEEVKGKALASFVKLNNKINAHGRMSFNGVSVHKNDFLRLARILSNEAVGLVLGGGGSRGISHVGVVIALEKHGIPIDLVGGTSIGSFVGGLYAMDYNTVSIYGRAKKFAKRVSSFWRILTDLTYPVTSYMTGYEFNRGIWKIFGFTEIEDFWLRYFCNSTNITNSTMDIHETGYSWRFIRASMSLAGLLPPITYNGSMLLDGGYLDNLPVSEMKKKGAKYIIAVDVGSVDDRTPMNYGDTLSGFWVLLNRWNPFSKHPDVPNMMDIQLRLAYVASVNALEISKKTPGVMYLRPPIENYATLDFAKYDEIYRVGYVYADELFTSWKSSGKLPDIAGMGDKVRKDMSGEHVSLSRRNSI |
| Enzyme Length | 1438 |
| Uniprot Accession Number | A5DHA3 |
| Absorption | |
| Active Site | ACT_SITE 1164; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1282; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 590..720; /note=cNMP 1; NP_BIND 707..856; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Domain (1); Motif (3); Nucleotide binding (2); Region (1); Topological domain (3); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1135..1140; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1162..1166; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1282..1284; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 160,000 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |