| IED ID | IndEnz0010000442 |
| Enzyme Type ID | esterase000442 |
| Protein Name |
Iron III enterobactin esterase EC 3.1.1.108 |
| Gene Name | pfeE PA2689 |
| Organism | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas aeruginosa group Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Enzyme Sequence | MRTSLLVAALGLALAAALPGGAPLAQPDPEATMDRSLLQRQDLPYRFSAVDLDSVDGQRHYRLWLGRPLQAPPAAGYPVVWMLDGNAAVGALDESTLRRLADGDAPLLVAIGYRTPLRIDRAGRTFDYTPASPGQADQRDPLNGLPSGGADAFLDLLRDGMRPAVAAQAPLDTARQTLWGHSYGGLLVLHALFTRPGEFARYAAASPSLWWRDGAILGERAGLEQRLRGKRAELLLWRGSAEPASPRGSLKAEPGQAMARLVDDLRRVAGLTLDFQPLDGLGHGETLGASLRLLLARPAVERQR |
| Enzyme Length | 304 |
| Uniprot Accession Number | Q9I0F2 |
| Absorption | |
| Active Site | ACT_SITE 182; /note=Charge relay system; /evidence=ECO:0000305|PubMed:30086222; ACT_SITE 242; /note=Charge relay system; /evidence=ECO:0000305|PubMed:30086222; ACT_SITE 283; /note=Charge relay system; /evidence=ECO:0000305|PubMed:30086222 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Fe(III)-enterobactin + H(+) + 3 H2O = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine] + 2 N-(2,3-dihydroxybenzoyl)-L-serine; Xref=Rhea:RHEA:30111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28199, ChEBI:CHEBI:58154, ChEBI:CHEBI:143010; EC=3.1.1.108; Evidence={ECO:0000269|PubMed:30086222};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30112; Evidence={ECO:0000269|PubMed:30086222}; CATALYTIC ACTIVITY: Reaction=Fe(III)-enterobactin + H2O = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]3 + H(+); Xref=Rhea:RHEA:59256, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28199, ChEBI:CHEBI:143011; Evidence={ECO:0000269|PubMed:30086222};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59257; Evidence={ECO:0000269|PubMed:30086222}; CATALYTIC ACTIVITY: Reaction=Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]3 + H(+) + H2O = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]2 + N-(2,3-dihydroxybenzoyl)-L-serine; Xref=Rhea:RHEA:59260, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58154, ChEBI:CHEBI:143011, ChEBI:CHEBI:143012; Evidence={ECO:0000269|PubMed:30086222};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59261; Evidence={ECO:0000269|PubMed:30086222}; CATALYTIC ACTIVITY: Reaction=Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]2 + H(+) + H2O = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine] + N-(2,3-dihydroxybenzoyl)-L-serine; Xref=Rhea:RHEA:59264, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58154, ChEBI:CHEBI:143010, ChEBI:CHEBI:143012; Evidence={ECO:0000269|PubMed:30086222};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59265; Evidence={ECO:0000269|PubMed:30086222}; |
| DNA Binding | |
| EC Number | 3.1.1.108 |
| Enzyme Function | FUNCTION: Catalyzes the hydrolysis of ferric enterobactin (Fe-Ent). Hydrolyzes Fe-Ent into three molecules of 2,3-dihydroxybenzoylserine (DHBS) still complexed with ferric iron. Iron reduction is necessary to obtain complete release of the metal from DHBS. It can hydrolyze salmochelin S4 (diglucosyl-C-Ent) but is not involved in iron acquisition by this siderophore. {ECO:0000269|PubMed:30086222}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (11); Chain (1); Helix (10); Mutagenesis (2); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Hydrolase;Periplasm;Reference proteome;Serine esterase;Signal |
| Interact With | |
| Induction | INDUCTION: Expression is induced by the presence of enterobactin in bacterial environment. {ECO:0000269|PubMed:30086222}. |
| Subcellular Location | SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:30086222}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 6GI0; 6GI1; 6GI2; 6GI5; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 32,665 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:30111; RHEA:30112; RHEA:59256; RHEA:59257; RHEA:59260; RHEA:59261; RHEA:59264; RHEA:59265 |
| Cross Reference Brenda |