| IED ID | IndEnz0010000466 |
| Enzyme Type ID | esterase000466 |
| Protein Name |
Protein phosphatase methylesterase 1 PME-1 EC 3.1.1.89 |
| Gene Name | ppe1 SPBP4H10.17c |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MAFRKEELSQTLYENESEQSSETKELMLNNEESLSDWRNYFDEKLTIPGESGALNGTINGYLTLPQPDGCLLVLQHGAGSSAMSFAPVTQELLSNSDNKVGFLALDLRAHGETTLEPESDMSLETLSKDFTHAVSYVQRMFELDEKIILVGHSLGGAICAYCAFQKTIPNTSGLVVIDVVEGTAMEALGFMKTYLSNRPTSFKSIDDAISWHIKTLVTRNRLSACITVPSLLVQQEDGTFVWRTDLYKTSPYWMDWFKGLSDKFLRAPYGRMLIVAGTDRLDKTLTIGQMQGKYQLEILPETGHFVHEDVPAKISSLLLNFWHRNQPLVLPPKVGATPVLQ |
| Enzyme Length | 341 |
| Uniprot Accession Number | Q9P7D2 |
| Absorption | |
| Active Site | ACT_SITE 153; /evidence=ECO:0000250; ACT_SITE 178; /evidence=ECO:0000250; ACT_SITE 304; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + H(+) + methanol; Xref=Rhea:RHEA:48548, Rhea:RHEA-COMP:12134, Rhea:RHEA-COMP:12135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:90516, ChEBI:CHEBI:90517; EC=3.1.1.89; |
| DNA Binding | |
| EC Number | 3.1.1.89 |
| Enzyme Function | FUNCTION: Demethylates proteins that have been reversibly carboxymethylated. Demethylates the phosphatase PP2A catalytic subunit (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Region (1) |
| Keywords | Hydrolase;Reference proteome;Serine esterase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20473289; 23697806; 23861937; 34250083; |
| Motif | |
| Gene Encoded By | |
| Mass | 38,092 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:48548 |
| Cross Reference Brenda |