| IED ID | IndEnz0010000476 |
| Enzyme Type ID | esterase000476 |
| Protein Name |
Methylesterase 4 AtMES4 EC 3.1.1.- Alpha/beta fold hydrolase/esterase 4 |
| Gene Name | MES4 ABE4 At2g23580 F26B6.23 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MEKNNKKRFVLVHGLCHGAWCWYKVKTHLEAVGHCVTAVDLAASGINMTRLEEIQTLKDYCKPLLELLNSLGSDDDKVILVAHSMGGIPAALASDIFPSKIATIVFLTAFMPDTRNLPAYVYQKLIRSVPQEGWLDTVFGTYGKHECPLEFALFGPKFMAKNLYQLSPVQDLELAKMLVRVNPIITNNLAGTRSFSEEGYGTVTRIYIVCGEDMAVPEDYQWWMIKNFPPKEVMEIKCADHMAMFSKPHKLCALLVEIACKYA |
| Enzyme Length | 263 |
| Uniprot Accession Number | O80474 |
| Absorption | |
| Active Site | ACT_SITE 84; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 213; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0; ACT_SITE 241; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q6RYA0 |
| Activity Regulation | ACTIVITY REGULATION: Esterase activity is down-regulated by salicylic acid (SA). {ECO:0000269|PubMed:18643994}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + methyl salicylate = H(+) + methanol + salicylate; Xref=Rhea:RHEA:33611, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:30762, ChEBI:CHEBI:31832; Evidence={ECO:0000269|PubMed:18467465};PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33612; Evidence={ECO:0000269|PubMed:18467465}; |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Methylesterase shown to have carboxylesterase activity and methyl salicylate (MeSA) esterase activity in vitro. {ECO:0000269|PubMed:18467465, ECO:0000269|PubMed:18643994}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Plant hormone biosynthesis. {ECO:0000269|PubMed:18467465}. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1) |
| Keywords | Alternative splicing;Hydrolase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 19136646; 21798944; 23959884; |
| Motif | |
| Gene Encoded By | |
| Mass | 29,582 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: Vmax=16.07 nmol/min/ug enzyme with methyl salicylate (MeSA) as substrate {ECO:0000269|PubMed:18643994}; |
| Metal Binding | |
| Rhea ID | RHEA:33611; RHEA:33612 |
| Cross Reference Brenda |