| IED ID | IndEnz0010000488 |
| Enzyme Type ID | esterase000488 |
| Protein Name |
Lysophospholipase NTE1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | NTE1 KLLA0B05225g |
| Organism | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Kluyveromyces Kluyveromyces lactis (Yeast) (Candida sphaerica) Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) |
| Enzyme Sequence | MWLTSYVLPRLKNILLLQFHITLPLNYLVLLLLSTVIITYLFLRTRILSNYSQLKDDVSDENNINRKDYMDASSASFLLNREKHKGFTSYLDEFLSAIKIFGYLEKPVFHELTKSMKTEKLQEGEIVLLDDSVGFTIVVEGTLEILHKMDNRHSNPSGDAAANATAFEPPSNDDGYLINGEKFQLLNIVKTGNPLSSLVSIMKLFSNRSTHLNEGTHPSTTNNTPGPGSPNLTGEINSFLDSTLPAKFGSADSANGNHPISPMELESTFDNLSEASENDRSAKIPDIIARAASDCTIAIIPSSSFQRLLVKYPRSASHIIQMILTKLYRVTFKTAHTYLGLTNEIIFTEFQSINKDNLKLPEYFRRSIISYFTNRQDDTGSSASTIQKRPQLHRRDSNNSLNYGSRHVVLNSRDQYNPGDLLSNVPLPRLNPQQRNDLSNTNSSSTLSRADYRPIILNNFSSSQFEETEVSSWRLALVEIIFQQLGITKDTIEPPISDDFSLHDHSLDEKGLVRRSSYSSFTSLSSSIATHSSNHLVTFLPRESQQFSRLNRTGGKMASHSKRLNGSSRSNSRTDRSESFDHFRNENLGGDNQFSDFESVKEDFSKCIKILKFEEGETILCQNSNPQGIYYLVSGEVDVISETKDGNTDESYERTLYTATEGYILGYLSSILGCKSLVTLKVSKGPAYLGLIPYNDLERLCDKYFMIYLKLSEILTNSLSPNLLRLDYFLEWIQLDSSETLFNQGDPANGVYLVLNGRLRQLFYEDADSDIVTQMAELSKGESFGEVEVLTAIHRLNTVVAIRDTELARIPRTLFEFLAVEHPSIMIHVSRMVAKKAMLMNFKSGIGFSGSQPITKLIGDETAMQKRYDFNLNIKSNKSSKKNELISNTVNYKTLTLLPITEGLPVEEFAYKLINALRQCGKTTIGLNQRTTLSHLGRHAFNKLSKLKQSGYFAELEELYEIVVYIADTPVSSSWTQTCISQGDCILLLADATCDPKIGEFERLLLKSKTTARTDLILLHPERYVVPGSTSKWLKNRMWIQSHHHIQFTPMEKVAEPDPIPNIKPLSQLVEKFKENTKKTQENFVKFLPDSIKTTVETLSGKYIDPKPNPKFYTSVDPIKNDFLRLARTLSGQAVGLVLGGGGARGLSHLGIIKALEEQGIPIDIIGGTSIGSFVGGLYAMDYDLVPIYGRVKKFAGRVGSLWRMLSDLTWPVTSYTTGHEFNRGIWKSFRDYRIEDFWISYYCNSTNITESVQEIHSSGFAWRYIRASMSLAGLLPPIVDNGNMLLDGGYVDNLPVTEMTQRGCKIVFAVDVGSVDDRTPMSYGDSLNGFWIVLNRWNPFSKHPNIPNMAEIQMRLGYVASVNALERAKSTPGVVYIRPPIENYATLDFGKFEEIYQVGYAYGHDFLQHLQEKNELPPIAGTTTSAYADKENMLQRRNSI |
| Enzyme Length | 1441 |
| Uniprot Accession Number | Q6CWC2 |
| Absorption | |
| Active Site | ACT_SITE 1170; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1288; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 592..718; /note=cNMP 1; NP_BIND 707..836; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Compositional bias (3); Domain (1); Motif (3); Nucleotide binding (2); Region (4); Topological domain (2); Transmembrane (1) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1141..1146; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1168..1172; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1288..1290; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 162,157 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |