| IED ID | IndEnz0010000489 |
| Enzyme Type ID | esterase000489 |
| Protein Name |
Lysophospholipase NTE1 EC 3.1.1.5 Intracellular phospholipase B Neuropathy target esterase homolog |
| Gene Name | NTE1 LELG_05397 |
| Organism | Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Debaryomycetaceae Candida/Lodderomyces clade Lodderomyces Lodderomyces elongisporus Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus) |
| Enzyme Sequence | MEEELAIEDLPRLTGTVSLNNGLLHSIYNETTVFKILRWSLVEIPKYILKLMSKNLEINLNVSSILIITLLIAAGILVIVRYKFLTGYSEDIKKGGSNANTKALGQQSTNYPKSTSSGLFVEKSKDKKPSNYLDEFLMAIKVFGYLDKQVFHELTKSMTTQKLSRDEVMCLDEKIGFLIVVEGTAQVYTKVTKKSNRNKYDELGQNGRDEGEEADEDDEEEEKEVGDDGDDEMDVEAMRNRGNGTKNGKMHGLDTDNFEENDDFLKLGEQNYQLLNEVKSGAALSSLISTLDLFKPLKSESTLTPLDSMGSGVSLNLDYLDRAGARLKALENDSDADTRDSTYPDIIVRPKKKKHSDTITVAIIPQSAFERVQMKYPKSTSHIVTMVLTRLYKVTMTTIHNYLGLTGEIFKLEIELNKSCELGLPRYLVDGLIERLSQGGSNERKQQAKHFRRSPLERTQSRYVLLNSRVKSNNPGDLLSSVPISRDDPKLRVNKSSAPTLVDGSNRINFTDNIEETEENSLRIAIIENIFKIVGINEAQNLPHEQAPFRSLNSSANSSSIALNSPGYFSPNLPATTGRHHNVLKFSSNDSLMNTISLSQLKSQKTHSISRGSTTQKQLYKRRNPITEMNIKDAFAKVMELKYIEPNTTVVQQNSVFCGLYYVINGSLEVHYKQAETYSKSATSKHVYTVGAGGIAGYMSCVVGFRSLVSIKTPKKTGAVVAYIAKNDYNQLLDKFYFLQLPMATKLKSLLSKQVMTIDYALEWCHIPAGNVLCSQGDLANGFHVVLSGRFRVVRKEKRKGSNRDEVKVLGEYGHGESIGEVEVLTASRRSNSLIAVRDSETARIPRTLFEILSFQNPSIMVKVSRLVASKVLSSEKTLSQATHSFITSSSNESFISADYKTITILPTVSGLPVRDFADKLVHSLKAIGRNVIALDQALTLTHLGRHAFDESLVRLKLSGYFAYLEEEYETVVYICDTPVQSNWTSTCISQGDCVLLLADADDQYTASSVGEYEQLLIKMKTTARTDLCLIHQEKFVVSGSTSRWLKNRMWVQGHHHIQMYIERNNETIGPGKKSFINEMAAKFVQNKSLISKFEEARSKALSWRREEQLKDLTLGSHKSDFLRLARILSNEAVGLVLGGGGSRGISHVGVVTALERHGIPVDLIGGTSIGSFVGGLYAKDYNIVSIYGRAKKFSKRVSSVWRMIFDLTYPVTSYITGYEFNRGIWKVFGFTEIEDFWIKYFCNSTNITNSTMDIHESGYAWRFIRASMSLAGLLPPIAFKGCMLLDGGYLDNLPVMEMKRRGAKHIFAVDVGSVDDRTPMDYGDTLSGFWVVFNKWNPFSKHPNVPNMMDIQLRLAYVASVNALEEAKRTPGVYYLRPPIDNYATLDFGKFDEIYQVGLGYADKLFTEWENKKQLPEIAGFVKRDGMQNGGERIKMYRRNTM |
| Enzyme Length | 1443 |
| Uniprot Accession Number | A5E708 |
| Absorption | |
| Active Site | ACT_SITE 1169; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 1287; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by organophosphorus esters. {ECO:0000250}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid + H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870, ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5; |
| DNA Binding | |
| EC Number | 3.1.1.5 |
| Enzyme Function | FUNCTION: Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 619..750; /note=cNMP 1; NP_BIND 746..871; /note=cNMP 2 |
| Features | Active site (2); Chain (1); Compositional bias (2); Domain (1); Motif (3); Nucleotide binding (2); Region (2); Topological domain (2); Transmembrane (1) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 1140..1145; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1167..1171; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 1287..1289; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 161,900 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:15177 |
| Cross Reference Brenda |