Detail Information for IndEnz0010000498
IED ID IndEnz0010000498
Enzyme Type ID esterase000498
Protein Name Pectinesterase
PE
EC 3.1.1.11
Pectin methylesterase
Gene Name pme RSp0138 RS02982
Organism Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Ralstonia Ralstonia solanacearum (Pseudomonas solanacearum) Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Enzyme Sequence MQSKTLYLKATALLGGCTVFAATALAVTSTATRPQLSSADARTYTIAKYLASFGTIGSLTTDNWDPTGGVGAVSGFRANYAVAADGTAQYKTVQAAIDAAVNAGGVARRYISVKAGIYNELVCVPESAPPITLYGLDANAGNTQIVYNNANPTPASGAKTNPCMGTSSNATVGTLRSATAMVRASDFHARNLTFKNSYVEGTYADNNQSAVALAVRGDKAILENVSVIGNQDTLFVGATSTTTVIRAYFKNSFIQGDTDFIFGAGTAVFHGCTIQYTAARLGAKAASYIFAPSTAPGNPYGFLAINSTFNATGNPPNNSLHLGRAWDQSVSGTSAYINGSSPNGQVVIRDSSLGALIRLADPWGPSTAGRPYCSANCAYSANRFFEYNNTGAGSGN
Enzyme Length 396
Uniprot Accession Number P58601
Absorption
Active Site ACT_SITE 232; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040; ACT_SITE 259; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10040
Activity Regulation
Binding Site BINDING 174; /note=Substrate; /evidence=ECO:0000250; BINDING 324; /note=Substrate; /evidence=ECO:0000250; BINDING 326; /note=Substrate; /evidence=ECO:0000250
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1->4)-alpha-D-galacturonosyl](n) + n H(+) + n methanol; Xref=Rhea:RHEA:22380, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:140522, ChEBI:CHEBI:140523; EC=3.1.1.11;
DNA Binding
EC Number 3.1.1.11
Enzyme Function FUNCTION: Involved in maceration and soft-rotting of plant tissue. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
nucleotide Binding
Features Active site (2); Binding site (3); Chain (1); Erroneous initiation (1); Signal peptide (1); Site (1)
Keywords Aspartyl esterase;Cell wall biogenesis/degradation;Hydrolase;Plasmid;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By Plasmid megaplasmid Rsp
Mass 40,961
Kinetics
Metal Binding
Rhea ID RHEA:22380
Cross Reference Brenda